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. 1969 Mar;62(3):764–771. doi: 10.1073/pnas.62.3.764

ACTIVE TRANSPORT OF MAGNESIUM IN Escherichia coli*

Simon Silver 1
PMCID: PMC223664  PMID: 4895213

Abstract

Magnesium-28 is accumulated by cells of Escherichia coli by a process that is temperature-dependent and inhibited by dinitrophenol and cyanide. The rate of uptake of magnesium is concentration-dependent, with a “Km” of about 5 × 10-4M in tryptone broth. There is evidence for cellular control over this rate. The magnesium accumulation system is specific, since neither potassium nor calcium (nor apparently manganese) competes with magnesium for cellular uptake. The magnesium that is accumulated by the cells can be released by treatment with toluene and partially exchanges with excess exogenous magnesium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROCK T. D. Effects of magnesium ion deficiency on Escherichia coli and possible relation to the mode of action of novobiocin. J Bacteriol. 1962 Oct;84:679–682. doi: 10.1128/jb.84.4.679-682.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cota-Robles E. H. Internal membranes in cells of Escherichia coli. J Ultrastruct Res. 1966 Dec;16(5):626–639. doi: 10.1016/s0022-5320(66)80010-2. [DOI] [PubMed] [Google Scholar]
  3. Damadian R. Ion metabolism in a potassium accumulation mutant of Escherichia coli B. I. Potassium metabolism. J Bacteriol. 1968 Jan;95(1):113–122. doi: 10.1128/jb.95.1.113-122.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dreyfuss J., Pardee A. B. Regulation of sulfate transport in Salmonella typhimurium. J Bacteriol. 1966 Jun;91(6):2275–2280. doi: 10.1128/jb.91.6.2275-2280.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Epstein W., Schultz S. G. Cation transport in Escherichia coli. VI. K exchange. J Gen Physiol. 1966 Jan;49(3):469–481. doi: 10.1085/jgp.49.3.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hurwitz C., Rosano C. L. The intracellular concentration of bound and unbound magnesium ions in Escherichia coli. J Biol Chem. 1967 Aug 25;242(16):3719–3722. [PubMed] [Google Scholar]
  7. Jackson R. W., DeMoss J. A. Effects of toluene on Escherichia coli. J Bacteriol. 1965 Nov;90(5):1420–1425. doi: 10.1128/jb.90.5.1420-1425.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kennell D., Kotoulas A. Magnesium starvation of Aerobacter aerogenes. I. Changes in nucleic acid composition. J Bacteriol. 1967 Jan;93(1):334–344. doi: 10.1128/jb.93.1.334-344.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lusk J. E., Williams R. J., Kennedy E. P. Magnesium and the growth of Escherichia coli. J Biol Chem. 1968 May 25;243(10):2618–2624. [PubMed] [Google Scholar]
  10. Sekiguchi M. Studies on the physiological defect in rII mutants of bacteriophage T4. J Mol Biol. 1966 Apr;16(2):503–522. doi: 10.1016/s0022-2836(66)80188-2. [DOI] [PubMed] [Google Scholar]
  11. Silver S., Kralovic M. L. Manganese accumulation by Escherichia coli: evidence for a specific transport system. Biochem Biophys Res Commun. 1969 Mar 10;34(5):640–645. doi: 10.1016/0006-291x(69)90786-4. [DOI] [PubMed] [Google Scholar]
  12. Silver S., Levine E. Action of steroidal diamines on active transport and permeability properties of Escherichia coli. J Bacteriol. 1968 Aug;96(2):338–345. doi: 10.1128/jb.96.2.338-345.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Silver S., Levine E., Spielman P. M. Cation fluxes and permeability changes accompanying bacteriophage infection of Escherichia coli. J Virol. 1968 Aug;2(8):763–771. doi: 10.1128/jvi.2.8.763-771.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Silver S., Wendt L. Mechanism of action of phenethyl alcohol: breakdown of the cellular permeability barrier. J Bacteriol. 1967 Feb;93(2):560–566. doi: 10.1128/jb.93.2.560-566.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tempest D. W., Dicks J. W., Meers J. L. Magnesium-limited growth of Bacillus subtilis, in pure and mixed cultures, in a chemostat. J Gen Microbiol. 1967 Oct;49(1):139–147. doi: 10.1099/00221287-49-1-139. [DOI] [PubMed] [Google Scholar]
  16. Webb M. The utilization of magnesium by certain Gram-positive and Gram-negative bacteria. J Gen Microbiol. 1966 Jun;43(3):401–409. doi: 10.1099/00221287-43-3-401. [DOI] [PubMed] [Google Scholar]

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