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. 1969 Mar;62(3):881–886. doi: 10.1073/pnas.62.3.881

ORIGIN AND DIRECTION OF DNA SYNTHESIS IN E. coli B/r*

C B Ward 1,†, D A Glaser 1
PMCID: PMC223680  PMID: 4895216

Abstract

Replication of the chromosome of E. coli B/r has been studied by measuring the change with cell age of the rates of reversion to wild type of auxotrophic markers induced by nitrosoguanidine. Synchronous populations of bacteria were obtained by the procedure of Helmstetter and Cummings. The origin of DNA synthesis was determined to occur at 49 minutes ± 5 minutes on the genetic map, and the direction of synthesis was found to be clockwise.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Tomizawa J. Replication of the escherichia coli K12 chromosome. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1911–1918. doi: 10.1073/pnas.58.5.1911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BONHOEFFER F., GIERER A. ON THE GROWTH MECHANISM OF THE BACTERIAL CHROMOSOME. J Mol Biol. 1963 Nov;7:534–540. doi: 10.1016/s0022-2836(63)80100-x. [DOI] [PubMed] [Google Scholar]
  3. CAIRNS J. The bacterial chromosome and its manner of replication as seen by autoradiography. J Mol Biol. 1963 Mar;6:208–213. doi: 10.1016/s0022-2836(63)80070-4. [DOI] [PubMed] [Google Scholar]
  4. Donachie W. D., Masters M. Evidence for polarity of chromosome replication in F- strains of Escherichia coli. Genet Res. 1966 Aug;8(1):119–124. doi: 10.1017/s0016672300009976. [DOI] [PubMed] [Google Scholar]
  5. HELMSTETTER C. E., CUMMINGS D. J. BACTERIAL SYNCHRONIZATION BY SELECTION OF CELLS AT DIVISION. Proc Natl Acad Sci U S A. 1963 Oct;50:767–774. doi: 10.1073/pnas.50.4.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helmstetter C. E. Origin and sequence of chromosome replication in Escherichia coli B-r. J Bacteriol. 1968 May;95(5):1634–1641. doi: 10.1128/jb.95.5.1634-1641.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  8. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  9. Low B. Inversion of transfer modes and sex factor-chromosome interactions in conjugation in Escherichia coli. J Bacteriol. 1967 Jan;93(1):98–106. doi: 10.1128/jb.93.1.98-106.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NAGATA T. The molecular synchrony and sequential replication of DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1963 Apr;49:551–559. doi: 10.1073/pnas.49.4.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nishi A., Horiuchi T. Beta-galactosidase formation controlled by an episomal gene during the cell cycle of Escherichia coli. J Biochem. 1966 Sep;60(3):338–340. doi: 10.1093/oxfordjournals.jbchem.a128442. [DOI] [PubMed] [Google Scholar]
  13. Pato M. L., Glaser D. A. The origin and direction of replication of the chromosome of Escherichia coli B-r. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1268–1274. doi: 10.1073/pnas.60.4.1268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rudner R., Rejman E., Chargaff E. Genetic implications of periodic pulsations of the rate of synthesis and the composition of rapidly labeled bacterial RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):904–911. doi: 10.1073/pnas.54.3.904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolf B., Newman A., Glaser D. A. On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968 Mar 28;32(3):611–629. doi: 10.1016/0022-2836(68)90346-x. [DOI] [PubMed] [Google Scholar]

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