Abstract
Several aspects of the strA phenotype were studied in strains of Escherichia coli diploid in the strA chromosomal region. It was found that alleles causing different levels of interference with amber suppression can complement each other, the less restrictive effects being predominant. The sensitive strA+ allele determines two responses to streptomycin: a dominant effect consisting of a sudden, complete, but reversible inhibition of growth, and a recessive effect manifested as cell killing. Both restriction of suppression and inhibition of growth reflect ribosomal involvement in translation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brownstein B. L., Lewandowski L. J. A mutation suppressing streptomycin dependence. I. An effect on ribosome function. J Mol Biol. 1967 Apr 14;25(1):99–109. doi: 10.1016/0022-2836(67)90281-1. [DOI] [PubMed] [Google Scholar]
- CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Couturier M., Desmet L., Thomas R. High pleiotropy of streptomycin mutations in Escherichia coli. Biochem Biophys Res Commun. 1964 Jun 15;16(3):244–248. doi: 10.1016/0006-291x(64)90333-x. [DOI] [PubMed] [Google Scholar]
- GORINI L., GUNDERSEN W., BURGER M. Genetics of regulation of enzyme synthesis in the arginine biosynthetic pathway of Escherichia coli. Cold Spring Harb Symp Quant Biol. 1961;26:173–182. doi: 10.1101/sqb.1961.026.01.022. [DOI] [PubMed] [Google Scholar]
- GORINI L., KATAJA E. STREPTOMYCIN-INDUCED OVERSUPPRESSION IN E. COLI. Proc Natl Acad Sci U S A. 1964 Jun;51:995–1001. doi: 10.1073/pnas.51.6.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gartner T. K., Orias E. Effects of mutations to streptomycin resistance on the rate of translation of mutant genetic information. J Bacteriol. 1966 Mar;91(3):1021–1028. doi: 10.1128/jb.91.3.1021-1028.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gorini L., Jacoby G. A., Breckenridge L. Ribosomal ambiguity. Cold Spring Harb Symp Quant Biol. 1966;31:657–664. doi: 10.1101/sqb.1966.031.01.084. [DOI] [PubMed] [Google Scholar]
- Gorini L., Rosset R., Zimmermann R. A. Phenotype masking and streptomycin dependence. Science. 1967 Sep 15;157(3794):1314–1317. doi: 10.1126/science.157.3794.1314. [DOI] [PubMed] [Google Scholar]
- LEDERBERG J. Streptomycin resistance; a genetically recessive mutation. J Bacteriol. 1951 May;61(5):549–550. doi: 10.1128/jb.61.5.549-550.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luzzatto L., Apirion D., Schlessinger D. Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis. Proc Natl Acad Sci U S A. 1968 Jul;60(3):873–880. doi: 10.1073/pnas.60.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosset R., Gorini L. A ribosomal ambiguity mutation. J Mol Biol. 1969 Jan 14;39(1):95–112. doi: 10.1016/0022-2836(69)90336-2. [DOI] [PubMed] [Google Scholar]
- Sparling P. F., Modolell J., Takeda Y., Davis B. D. Ribosomes from Escherichia coli merodiplods heterozygous for resistance to streptomycin and to spectinomycin. J Mol Biol. 1968 Nov 14;37(3):407–421. doi: 10.1016/0022-2836(68)90111-3. [DOI] [PubMed] [Google Scholar]
- Traub P., Nomura M. Streptomycin resistance mutation in Escherichia coli: altered ribosomal protein. Science. 1968 Apr 12;160(3824):198–199. doi: 10.1126/science.160.3824.198. [DOI] [PubMed] [Google Scholar]