Abstract
1. Sodium dodecyl sulfate (SDS) attacks the chlorophyll-protein compound modifying its protein properties and absorption spectrum. 2. In the presence of SDS, chlorophyll is quantitatively converted to phaeophytin; i.e., magnesium is removed from the molecule. This reaction, measured spectrophotometrically, proceeds at a rate directly proportional to the hydrogen ion concentration. At constant pH, the rate is proportional to the SDS concentration until a maximum rate is achieved. 3. The chlorophyll or phaeophytin (depending on the pH) remains attached to the protein, since the prosthetic group cannot be separated by ultrafiltration, dialysis, or fractional precipitation. 4. This suggests that the magnesium plays no part in binding chlorophyll to the split protein fragments, but may be concerned in binding the larger units, since the metal becomes extremely labile when the protein is split.
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Selected References
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- Inman O. L., Crowell M. L. CONDITION OF CHLOROPHYLL IN THE LEAF. Plant Physiol. 1939 Apr;14(2):388–390. doi: 10.1104/pp.14.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith E. L. CHLOROPHYLL AS THE PROSTHETIC GROUP OF A PROTEIN IN THE GREEN LEAF. Science. 1940 Feb 23;91(2356):199–200. doi: 10.1126/science.91.2356.199. [DOI] [PubMed] [Google Scholar]
- Smith E. L. THE CHLOROPHYLL-PROTEIN COMPOUND OF THE GREEN LEAF. J Gen Physiol. 1941 May 20;24(5):565–582. doi: 10.1085/jgp.24.5.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sreenivasaya M., Pirie N. W. The disintegration of tobacco mosaic virus preparations with sodium dodecyl sulphate. Biochem J. 1938 Oct;32(10):1707–1710. doi: 10.1042/bj0321707. [DOI] [PMC free article] [PubMed] [Google Scholar]