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. 1988 Apr;57(4):353–357. doi: 10.1038/bjc.1988.81

Vimentin and p53 expression on epidermal growth factor receptor-positive, oestrogen receptor-negative breast carcinomas.

G Cattoretti 1, S Andreola 1, C Clemente 1, L D'Amato 1, F Rilke 1
PMCID: PMC2246580  PMID: 3291921

Abstract

The coordinate expression of the nuclear p53 protein, cytoplasmic intermediate filament vimentin (VIM) and membrane epidermal growth factor receptor (EGF-R) was significantly associated with oestrogen receptor immunocytochemical nuclear stain (ER-ICA) negative breast carcinomas. Twenty-three (51.1%), 26 (57.8%) and 27 (60%) of 45 ER-ICA -ve cancers were respectively p53 +ve, VIM +ve and EGF-R +ve; whereas of 151 ER-ICA +ve tumours 8 (5.3%) were p53 +ve (P less than 0.0001), 23 (15.2%) VIM +ve (P less than 0.001) and 40 (26.5%) EGF-R +ve P less than 0.001). Thirty-six of 45 (80%) ER-ICA -ve carcinomas were positive for at least one of the markers versus 55/151 (36.4%) ER-ICA +ve cases (chi 2 = 28.92, P less than 0.001). A prevalence of high grade carcinomas was found among p53 +ve, VIM +ve cases; the latter subset of tumours also had a larger mean diameter. These results suggest that ER -ve breast carcinoma cells display a coordinate expression of cell cycle-related proteins and marked changes of both the cytoskeleton and the membrane receptor repertoire.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azumi N., Battifora H. The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. A comprehensive immunohistochemical study on formalin- and alcohol-fixed tumors. Am J Clin Pathol. 1987 Sep;88(3):286–296. doi: 10.1093/ajcp/88.3.286. [DOI] [PubMed] [Google Scholar]
  2. BLOOM H. J., RICHARDSON W. W. Histological grading and prognosis in breast cancer; a study of 1409 cases of which 359 have been followed for 15 years. Br J Cancer. 1957 Sep;11(3):359–377. doi: 10.1038/bjc.1957.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ben-Ze'ev A. Cell-cell interaction and cell configuration related control of cytokeratins and vimentin expression in epithelial cells and in fibroblasts. Ann N Y Acad Sci. 1985;455:597–613. doi: 10.1111/j.1749-6632.1985.tb50439.x. [DOI] [PubMed] [Google Scholar]
  4. Betsholtz C., Bergh J., Bywater M., Pettersson M., Johnsson A., Heldin C. H., Ohlsson R., Knott T. J., Scott J., Bell G. I. Expression of multiple growth factors in a human lung cancer cell line. Int J Cancer. 1987 Apr 15;39(4):502–507. doi: 10.1002/ijc.2910390417. [DOI] [PubMed] [Google Scholar]
  5. Bienz B., Zakut-Houri R., Givol D., Oren M. Analysis of the gene coding for the murine cellular tumour antigen p53. EMBO J. 1984 Sep;3(9):2179–2183. doi: 10.1002/j.1460-2075.1984.tb02110.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Braithwaite A. W., Sturzbecher H. W., Addison C., Palmer C., Rudge K., Jenkins J. R. Mouse p53 inhibits SV40 origin-dependent DNA replication. Nature. 1987 Oct 1;329(6138):458–460. doi: 10.1038/329458a0. [DOI] [PubMed] [Google Scholar]
  7. Cattoretti G., Rilke F., Andreola S., D'Amato L., Delia D. P53 expression in breast cancer. Int J Cancer. 1988 Feb 15;41(2):178–183. doi: 10.1002/ijc.2910410204. [DOI] [PubMed] [Google Scholar]
  8. Darzynkiewicz Z., Staiano-Coico L., Kunicka J. E., DeLeo A. B., Old L. J. p53 content in relation to cell growth and proliferation in murine L1210 leukemia and normal lymphocytes. Leuk Res. 1986;10(12):1383–1389. doi: 10.1016/0145-2126(86)90004-4. [DOI] [PubMed] [Google Scholar]
  9. Dickson R. B., McManaway M. E., Lippman M. E. Estrogen-induced factors of breast cancer cells partially replace estrogen to promote tumor growth. Science. 1986 Jun 20;232(4757):1540–1543. doi: 10.1126/science.3715461. [DOI] [PubMed] [Google Scholar]
  10. Downward J., Yarden Y., Mayes E., Scrace G., Totty N., Stockwell P., Ullrich A., Schlessinger J., Waterfield M. D. Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences. Nature. 1984 Feb 9;307(5951):521–527. doi: 10.1038/307521a0. [DOI] [PubMed] [Google Scholar]
  11. Eliyahu D., Raz A., Gruss P., Givol D., Oren M. Participation of p53 cellular tumour antigen in transformation of normal embryonic cells. Nature. 1984 Dec 13;312(5995):646–649. doi: 10.1038/312646a0. [DOI] [PubMed] [Google Scholar]
  12. Ferrari S., Battini R., Kaczmarek L., Rittling S., Calabretta B., de Riel J. K., Philiponis V., Wei J. F., Baserga R. Coding sequence and growth regulation of the human vimentin gene. Mol Cell Biol. 1986 Nov;6(11):3614–3620. doi: 10.1128/mcb.6.11.3614-3620.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Filmus J., Benchimol S., Buick R. N. Comparative analysis of the involvement of p53, c-myc and c-fos in epidermal growth factor-mediated signal transduction. Exp Cell Res. 1987 Apr;169(2):554–559. doi: 10.1016/0014-4827(87)90215-1. [DOI] [PubMed] [Google Scholar]
  14. Gould V. E. The coexpression of distinct classes of intermediate filaments in human neoplasms. Arch Pathol Lab Med. 1985 Nov;109(11):984–985. [PubMed] [Google Scholar]
  15. Henzen-Logmans S. C., Mullink H., Ramaekers F. C., Tadema T., Meijer C. J. Expression of cytokeratins and vimentin in epithelial cells of normal and pathologic thyroid tissue. Virchows Arch A Pathol Anat Histopathol. 1987;410(4):347–354. doi: 10.1007/BF00711291. [DOI] [PubMed] [Google Scholar]
  16. Jakesz R., Smith C. A., Aitken S., Huff K., Schuette W., Shackney S., Lippman M. Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells. Cancer Res. 1984 Feb;44(2):619–625. [PubMed] [Google Scholar]
  17. Kodama F., Greene G. L., Salmon S. E. Relation of estrogen receptor expression to clonal growth and antiestrogen effects on human breast cancer cells. Cancer Res. 1985 Jun;45(6):2720–2724. [PubMed] [Google Scholar]
  18. McGuire W. L., Clark G. M., Dressler L. G., Owens M. A. Role of steroid hormone receptors as prognostic factors in primary breast cancer. NCI Monogr. 1986;(1):19–23. [PubMed] [Google Scholar]
  19. McGuire W. L., Dressler L. G. Emerging impact of flow cytometry in predicting recurrence and survival in breast cancer patients. J Natl Cancer Inst. 1985 Sep;75(3):405–410. [PubMed] [Google Scholar]
  20. Mercer W. E., Baserga R. Expression of the p53 protein during the cell cycle of human peripheral blood lymphocytes. Exp Cell Res. 1985 Sep;160(1):31–46. doi: 10.1016/0014-4827(85)90233-2. [DOI] [PubMed] [Google Scholar]
  21. Mercer W. E., Nelson D., DeLeo A. B., Old L. J., Baserga R. Microinjection of monoclonal antibody to protein p53 inhibits serum-induced DNA synthesis in 3T3 cells. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6309–6312. doi: 10.1073/pnas.79.20.6309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Peres R., Betsholtz C., Westermark B., Heldin C. H. Frequent expression of growth factors for mesenchymal cells in human mammary carcinoma cell lines. Cancer Res. 1987 Jul 1;47(13):3425–3429. [PubMed] [Google Scholar]
  23. Ramaekers F. C., Haag D., Kant A., Moesker O., Jap P. H., Vooijs G. P. Coexpression of keratin- and vimentin-type intermediate filaments in human metastatic carcinoma cells. Proc Natl Acad Sci U S A. 1983 May;80(9):2618–2622. doi: 10.1073/pnas.80.9.2618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Reed J. C., Alpers J. D., Nowell P. C., Hoover R. G. Sequential expression of protooncogenes during lectin-stimulated mitogenesis of normal human lymphocytes. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3982–3986. doi: 10.1073/pnas.83.11.3982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rittling S. R., Brooks K. M., Cristofalo V. J., Baserga R. Expression of cell cycle-dependent genes in young and senescent WI-38 fibroblasts. Proc Natl Acad Sci U S A. 1986 May;83(10):3316–3320. doi: 10.1073/pnas.83.10.3316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sainsbury J. R., Farndon J. R., Needham G. K., Malcolm A. J., Harris A. L. Epidermal-growth-factor receptor status as predictor of early recurrence of and death from breast cancer. Lancet. 1987 Jun 20;1(8547):1398–1402. doi: 10.1016/s0140-6736(87)90593-9. [DOI] [PubMed] [Google Scholar]
  27. Schmid E., Schiller D. L., Grund C., Stadler J., Franke W. W. Tissue type-specific expression of intermediate filament proteins in a cultured epithelial cell line from bovine mammary gland. J Cell Biol. 1983 Jan;96(1):37–50. doi: 10.1083/jcb.96.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Silvestrini R., Daidone M. G., Bertuzzi A., Di Fronzo G. Relationship between estrogen receptors and cellular proliferation. Recent Results Cancer Res. 1984;91:163–168. doi: 10.1007/978-3-642-82188-2_23. [DOI] [PubMed] [Google Scholar]
  29. Taketani Y., Oka T. Biological action of epidermal growth factor and its functional receptors in normal mammary epithelial cells. Proc Natl Acad Sci U S A. 1983 May;80(9):2647–2650. doi: 10.1073/pnas.80.9.2647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Traub P. Are intermediate filament proteins involved in gene expression? Ann N Y Acad Sci. 1985;455:68–78. doi: 10.1111/j.1749-6632.1985.tb50404.x. [DOI] [PubMed] [Google Scholar]
  31. Walker R. A., Camplejohn R. S. DNA flow cytometry of human breast carcinomas and its relationship to transferrin and epidermal growth factor receptors. J Pathol. 1986 Sep;150(1):37–42. doi: 10.1002/path.1711500107. [DOI] [PubMed] [Google Scholar]

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