Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1988 Sep;58(3):301–305. doi: 10.1038/bjc.1988.208

The effect of fractionation of light treatment on necrosis and vascular function of normal skin following photodynamic therapy.

K Benstead 1, J V Moore 1
PMCID: PMC2246602  PMID: 3179181

Abstract

Sparing of normal tissue, mouse tail skin, by fractionation of light treatment in photodynamic therapy has been demonstrated in BDF1 mice injected with 2 mg tetrasodium-meso-tetra(4-sulphophenyl)porphine dodecahydrate i.v. When the time between 2 fractions of 67.5 J cm-2 and 90 J cm-2 was increased to 2 and 4 days respectively the incidence of necrosis fell to that expected after a single fraction. Blood flow in the tail skin 5 days after the second light fraction, as measured by the clearance of an intradermally injected solution of 133xenon in 0.9% saline, returned to control values when the time between 2 fractions was 2 days with 67.5 J cm-2 fractions, and 3 days with 90 J cm-2 fractions. The time course of recovery of normal mouse tail skin from photodynamic therapy, as shown by these split dose experiments, was found to be similar to the time course for the recovery of blood flow following a single light treatment.

Full text

PDF
301

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bellnier D. A., Lin C. W. Photosensitization and split-dose recovery in cultured human urinary bladder carcinoma cells containing nonexchangeable hematoporphyrin derivative. Cancer Res. 1985 Jun;45(6):2507–2511. [PubMed] [Google Scholar]
  2. Ben-Hur E., Kol R., Riklis E., Marko R., Rosenthal I. Effect of light fluence rate on mammalian cells photosensitization by chloroaluminium phthalocyanine tetrasulphonate. Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Mar;51(3):467–476. doi: 10.1080/09553008714550951. [DOI] [PubMed] [Google Scholar]
  3. Ben-Hur E., Rosenthal I. Photosensitized inactivation of Chinese hamster cells by phthalocyanines. Photochem Photobiol. 1985 Aug;42(2):129–133. doi: 10.1111/j.1751-1097.1985.tb01550.x. [DOI] [PubMed] [Google Scholar]
  4. Benson R. C., Jr Laser photodynamic therapy for bladder cancer. Mayo Clin Proc. 1986 Nov;61(11):859–864. doi: 10.1016/s0025-6196(12)62606-8. [DOI] [PubMed] [Google Scholar]
  5. Benstead K., Moore J. V. Vascular function and the probability of skin necrosis after photodynamic therapy: an experimental study. Br J Cancer. 1988 May;57(5):451–454. doi: 10.1038/bjc.1988.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Christensen T., Smedshammer L., Wahl A., Moan J. Photodynamic effects and hyperthermia in vitro. Adv Exp Med Biol. 1985;193:69–78. doi: 10.1007/978-1-4613-2165-1_8. [DOI] [PubMed] [Google Scholar]
  7. Christensen T., Wahl A., Smedshammer L. Effects of haematoporphyrin derivative and light in combination with hyperthermia on cells in culture. Br J Cancer. 1984 Jul;50(1):85–89. doi: 10.1038/bjc.1984.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dougherty T. J., Gomer C. J., Weishaupt K. R. Energetics and efficiency of photoinactivation of murine tumor cells containing hematoporphyrin. Cancer Res. 1976 Jul;36(7 Pt 1):2330–2333. [PubMed] [Google Scholar]
  9. Dougherty T. J. Photoradiation therapy for cutaneous and subcutaneous malignancies. J Invest Dermatol. 1981 Jul;77(1):122–124. doi: 10.1111/1523-1747.ep12479341. [DOI] [PubMed] [Google Scholar]
  10. Evensen J. F., Moan J., Winkelman J. W. Toxic and phototoxic effects of tetraphenylporphinesulphonate and haematoporphyrin derivative in vitro. Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Mar;51(3):477–491. doi: 10.1080/09553008714550961. [DOI] [PubMed] [Google Scholar]
  11. Gilbert C. W. Computer programmes for fitting Puck and probit survival curves. Int J Radiat Biol Relat Stud Phys Chem Med. 1969;16(4):323–332. doi: 10.1080/09553006914551351. [DOI] [PubMed] [Google Scholar]
  12. Gomer C. J., Rucker N., Murphree A. L. Examination of potentially lethal damage in cells treated with hematoporphyrin derivative and red light. Adv Exp Med Biol. 1985;193:147–153. doi: 10.1007/978-1-4613-2165-1_17. [DOI] [PubMed] [Google Scholar]
  13. Gomer C. J., Smith D. M. Photoinactivation of Chinese hamster cells by hematoporphyrin derivative and red light. Photochem Photobiol. 1980 Sep;32(3):341–348. doi: 10.1111/j.1751-1097.1980.tb03772.x. [DOI] [PubMed] [Google Scholar]
  14. KETY S. S. Measurement of regional circulation by the local clearance of radioactive sodium. Am Heart J. 1949 Sep;38(3):321–328. doi: 10.1016/0002-8703(49)90845-5. [DOI] [PubMed] [Google Scholar]
  15. Moan J., Christensen T. Photodynamic inactivation of cancer cells in vitro. Effect of irradiation temperature and dose fractionation. Cancer Lett. 1979 May;6(6):331–335. doi: 10.1016/s0304-3835(79)80090-7. [DOI] [PubMed] [Google Scholar]
  16. Moan J., Pettersen E. O., Christensen T. The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin. Br J Cancer. 1979 Apr;39(4):398–407. doi: 10.1038/bjc.1979.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moore J. V., Keene J. P., Land E. J. Dose-response relationships for photodynamic injury to murine skin. Br J Radiol. 1986 Mar;59(699):257–261. doi: 10.1259/0007-1285-59-699-257. [DOI] [PubMed] [Google Scholar]
  18. Moore J. V. Necrosis of murine tail skin following photodynamic treatment with meso-tetra-(p-sulphophenyl) porphine (TPPS). Photochem Photobiol. 1987 Jun;45(6):791–794. doi: 10.1111/j.1751-1097.1987.tb07884.x. [DOI] [PubMed] [Google Scholar]
  19. Soma H., Akiya K., Nutahara S., Kato H., Hayata Y. Treatment of vaginal carcinoma with laser photoirradiation following administration of haematoporphyrin derivative. Report of a case. Ann Chir Gynaecol. 1982;71(2):133–136. [PubMed] [Google Scholar]
  20. Ward B. G., Forbes I. J., Cowled P. A., McEvoy M. M., Cox L. W. The treatment of vaginal recurrences of gynecologic malignancy with phototherapy following hematoporphyrin derivative pretreatment. Am J Obstet Gynecol. 1982 Feb 1;142(3):356–357. doi: 10.1016/0002-9378(82)90744-x. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES