Abstract
Cell survival was investigated in an intraocular retinoblastoma-like tumour 30 min to 48 h after photodynamic therapy. The survival of the cells was assessed by an in vivo to in vitro colony forming assay, estimated by either the plating efficiency of the treated tumour cells compared to non-treated cells or the number of clonogenic cells per mg excised tumour. Curves showing cell survival as a function of the time between light irradiation and excision of the intraocular tumours were biphasic. This suggests more than one PDT tissue destruction mechanism in vivo (i.e. an early direct cell damage plus a subsequent late damage occurring in the tumour tissue left in situ after treatment). The delayed mechanism may be due to changes in the environment of the tumours probably caused by vascular damage. Tumour cells sensitised by Photofrin II in vivo and excised from the eyes were damaged by light when irradiated in vitro and this was dependent on the light energy dose. This showed that cellular Photofrin II uptake in the eye tumours was sufficient for direct cell damage and thus supports the suggestion that direct and indirect tumour destruction occurs in this eye tumour after photodynamic therapy.
Full text
PDF![869](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6030/2246719/84c1ec67f5c7/brjcancer00128-0037.png)
![870](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6030/2246719/e08dff353b9a/brjcancer00128-0038.png)
![871](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6030/2246719/d2eccef5c614/brjcancer00128-0039.png)
![872](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6030/2246719/b59759674049/brjcancer00128-0040.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berenbaum M. C., Hall G. W., Hoyes A. D. Cerebral photosensitisation by haematoporphyrin derivative. Evidence for an endothelial site of action. Br J Cancer. 1986 Jan;53(1):81–89. doi: 10.1038/bjc.1986.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bjerring P. Intratumoural light distribution in an experimental mouse tumour irradiated by a diffuse-light irradiator compared with unilateral helium-neon light for photodynamic therapy. Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Aug;52(2):191–199. doi: 10.1080/09553008714551651. [DOI] [PubMed] [Google Scholar]
- Bruce R. A., Jr Evaluation of hematoporphyrin photoradiation therapy to treat choroidal melanomas. Lasers Surg Med. 1984;4(1):59–64. doi: 10.1002/lsm.1900040108. [DOI] [PubMed] [Google Scholar]
- Bugelski P. J., Porter C. W., Dougherty T. J. Autoradiographic distribution of hematoporphyrin derivative in normal and tumor tissue of the mouse. Cancer Res. 1981 Nov;41(11 Pt 1):4606–4612. [PubMed] [Google Scholar]
- Franken K. A., van Delft J. L., Dubbelman T. M., de Wolff-Rouendaal D., Oosterhuis J. A., Star W. M., Marijnissen H. P. Hematoporphyrin derivative photoradiation treatment of experimental malignant melanoma in the anterior chamber of the rabbit. Curr Eye Res. 1985 May;4(5):641–654. doi: 10.3109/02713688508999997. [DOI] [PubMed] [Google Scholar]
- Gomer C. J., Jester J. V., Razum N. J., Szirth B. C., Murphree A. L. Photodynamic therapy of intraocular tumors: examination of hematoporphyrin derivative distribution and long-term damage in rabbit ocular tissue. Cancer Res. 1985 Aug;45(8):3718–3725. [PubMed] [Google Scholar]
- Henderson B. W., Waldow S. M., Mang T. S., Potter W. R., Malone P. B., Dougherty T. J. Tumor destruction and kinetics of tumor cell death in two experimental mouse tumors following photodynamic therapy. Cancer Res. 1985 Feb;45(2):572–576. [PubMed] [Google Scholar]
- Kobayashi M., Mukai N., Solish S. P., Pomeroy M. E. A highly predictable animal model of retinoblastoma. Acta Neuropathol. 1982;57(2-3):203–208. doi: 10.1007/BF00685390. [DOI] [PubMed] [Google Scholar]
- Marmor J. B., Hahn N., Hahn G. M. Tumor cure and cell survival after localized radiofrequency heating. Cancer Res. 1977 Mar;37(3):879–883. [PubMed] [Google Scholar]
- Moan J. Porphyrin photosensitization and phototherapy. Photochem Photobiol. 1986 Jun;43(6):681–690. doi: 10.1111/j.1751-1097.1986.tb05647.x. [DOI] [PubMed] [Google Scholar]
- Murphree A. L., Cote M., Gomer C. J. The evolution of photodynamic therapy techniques in the treatment of intraocular tumors. Photochem Photobiol. 1987 Nov;46(5):919–923. doi: 10.1111/j.1751-1097.1987.tb04869.x. [DOI] [PubMed] [Google Scholar]
- Nelson J. S., Liaw L. H., Berns M. W. Tumor destruction in photodynamic therapy. Photochem Photobiol. 1987 Nov;46(5):829–835. doi: 10.1111/j.1751-1097.1987.tb04855.x. [DOI] [PubMed] [Google Scholar]
- Ohnishi Y., Yamana Y., Minei M. Photoradiation therapy using argon laser and a hematoporphyrin derivative for retinoblastoma--a preliminary report. Jpn J Ophthalmol. 1986;30(4):409–419. [PubMed] [Google Scholar]
- Pepe I. M., Schwemer J. An improved HPLC method for the separation of retinaldehyde isomers from visual pigments. Photochem Photobiol. 1987 May;45(5):679–681. doi: 10.1111/j.1751-1097.1987.tb07400.x. [DOI] [PubMed] [Google Scholar]
- Sery T. W., Shields J. A., Augsburger J. J., Shah H. G. Photodynamic therapy of human ocular cancer. Ophthalmic Surg. 1987 Jun;18(6):413–418. [PubMed] [Google Scholar]
- Star W. M., Marijnissen H. P., van den Berg-Blok A. E., Versteeg J. A., Franken K. A., Reinhold H. S. Destruction of rat mammary tumor and normal tissue microcirculation by hematoporphyrin derivative photoradiation observed in vivo in sandwich observation chambers. Cancer Res. 1986 May;46(5):2532–2540. [PubMed] [Google Scholar]
- Waldow S. M., Dougherty T. J. Interaction of hyperthermia and photoradiation therapy. Radiat Res. 1984 Feb;97(2):380–385. [PubMed] [Google Scholar]
- Winther J. Effect of photofrin II and light energy on retinoblastoma-like cells in vitro. Dose-response relationships, effect of light dose rate and recovery ratio. J Cancer Res Clin Oncol. 1989;115(1):73–78. doi: 10.1007/BF00391603. [DOI] [PubMed] [Google Scholar]
- Winther J., Ehlers N. Histopathological changes in an intraocular retinoblastoma-like tumour following photodynamic therapy. Acta Ophthalmol (Copenh) 1988 Feb;66(1):69–78. doi: 10.1111/j.1755-3768.1988.tb08537.x. [DOI] [PubMed] [Google Scholar]
- Winther J. In vitro and in vivo growth of an intraocular retinoblastoma-like tumour in F-344 rats. Acta Ophthalmol (Copenh) 1986 Dec;64(6):657–663. doi: 10.1111/j.1755-3768.1986.tb00683.x. [DOI] [PubMed] [Google Scholar]
- Winther J., Jensen O. A., Prause J. U., Tommerup N. Characterization of an intraocular retinoblastoma-like tumour. Acta Ophthalmol (Copenh) 1987 Aug;65(4):491–502. doi: 10.1111/j.1755-3768.1987.tb07029.x. [DOI] [PubMed] [Google Scholar]
- Winther J., Overgaard J., Ehlers N. The effect of photodynamic therapy alone and in combination with misonidazole or X-rays for management of a retinoblastoma-like tumour. Photochem Photobiol. 1988 Mar;47(3):419–423. doi: 10.1111/j.1751-1097.1988.tb02746.x. [DOI] [PubMed] [Google Scholar]