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. 1988 Nov;58(5):600–605. doi: 10.1038/bjc.1988.267

Measurement of oestrogen receptor mRNA levels in human breast tumours.

J A Henry 1, S Nicholson 1, J R Farndon 1, B R Westley 1, F E May 1
PMCID: PMC2246829  PMID: 2851309

Abstract

A sensitive single-stranded hybridisation probe for the oestrogen receptor mRNA was synthesised using T3 polymerase from oestrogen receptor cDNA cloned in the Bluescript vector. This probe was used to measure oestrogen receptor mRNA in total RNA extracted from breast tumours. Oestrogen receptor mRNA was detected in 41 of 47 (87%) tumours whereas cytosolic oestrogen receptor protein was detected in only 18 out of 39 (46%). There was a significant correlation between the levels of the oestrogen receptor, as measured by 3H-oestradiol binding, and the oestrogen receptor mRNA. Oestrogen receptor mRNA was detected in 15 of the 21 tumours that did not contain detectable oestrogen receptor protein. This suggests that detection of the mRNA is a more sensitive means for establishing oestrogen receptor status than the radioligand oestrogen receptor assay. Oestrogen receptor mRNA was found in all histological tumour types examined. Its level was related to tumour differentiation. All the tumours that did not contain oestrogen receptor mRNA and most of the tumours that contained only low levels of oestrogen receptor mRNA were classified as grade III according to Bloom and Richardson: the median of the oestrogen receptor mRNA levels was significantly lower in this group.

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Selected References

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  1. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  2. BLOOM H. J., RICHARDSON W. W. Histological grading and prognosis in breast cancer; a study of 1409 cases of which 359 have been followed for 15 years. Br J Cancer. 1957 Sep;11(3):359–377. doi: 10.1038/bjc.1957.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barrett-Lee P. J., Travers M. T., McClelland R. A., Luqmani Y., Coombes R. C. Characterization of estrogen receptor messenger RNA in human breast cancer. Cancer Res. 1987 Dec 15;47(24 Pt 1):6653–6659. [PubMed] [Google Scholar]
  4. Castagnetta L., Traina A., Di Carlo A., Latteri A. M., Carruba G., Leake R. E. Heterogeneity of soluble and nuclear oestrogen receptor status of involved nodes in relation to primary breast cancer. Eur J Cancer Clin Oncol. 1987 Jan;23(1):31–35. doi: 10.1016/0277-5379(87)90415-9. [DOI] [PubMed] [Google Scholar]
  5. Coombes R. C., Powles T. J., Berger U., Wilson P., McClelland R. A., Gazet J. C., Trott P. A., Ford H. T. Prediction of endocrine response in breast cancer by immunocytochemical detection of oestrogen receptor in fine-needle aspirates. Lancet. 1987 Sep 26;2(8561):701–703. doi: 10.1016/s0140-6736(87)91071-3. [DOI] [PubMed] [Google Scholar]
  6. FOLCA P. J., GLASCOCK R. F., IRVINE W. T. Studies with tritium-labelled hexoestrol in advanced breast cancer. Comparison of tissue accumulation of hexoestrol with response to bilateral adrenalectomy and oophorectomy. Lancet. 1961 Oct 7;2(7206):796–798. doi: 10.1016/s0140-6736(61)91088-1. [DOI] [PubMed] [Google Scholar]
  7. Furmanski P., Saunders D. E., Brooks S. C., Rich M. A. The prognostic value of estrogen receptor determinations in patients with primary breast cancer: an update. Cancer. 1980 Dec 15;46(12 Suppl):2794–2796. doi: 10.1002/1097-0142(19801215)46:12+<2794::aid-cncr2820461410>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  8. Hawkins R. A. Receptors in the management of breast cancer. Br J Hosp Med. 1985 Sep;34(3):160–164. [PubMed] [Google Scholar]
  9. Hawkins R. A., White G., Bundred N. J., Dixon J. M., Miller W. R., Stewart H. J., Forrest A. P. Prognostic significance of oestrogen and progestogen receptor activities in breast cancer. Br J Surg. 1987 Nov;74(11):1009–1013. doi: 10.1002/bjs.1800741118. [DOI] [PubMed] [Google Scholar]
  10. Hull D. F., 3rd, Clark G. M., Osborne C. K., Chamness G. C., Knight W. A., 3rd, McGuire W. L. Multiple estrogen receptor assays in human breast cancer. Cancer Res. 1983 Jan;43(1):413–416. [PubMed] [Google Scholar]
  11. Korenman S. G., Dukes B. A. Specific estrogen binding by the cytoplasm fof human breast carcinoma. J Clin Endocrinol Metab. 1970 May;30(5):639–645. doi: 10.1210/jcem-30-5-639. [DOI] [PubMed] [Google Scholar]
  12. Leake R. E., Laing L., Calman K. C., Macbeth F. R., Crawford D., Smith D. C. Oestrogen-receptor status and endocrine therapy of breast cancer: response rates and status stability. Br J Cancer. 1981 Jan;43(1):59–66. doi: 10.1038/bjc.1981.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. May F. E., Westley B. R. Cloning of estrogen-regulated messenger RNA sequences from human breast cancer cells. Cancer Res. 1986 Dec;46(12 Pt 1):6034–6040. [PubMed] [Google Scholar]
  14. McCarty K. S., Jr, Barton T. K., Fetter B. F., Woodard B. H., Mossler J. A., Reeves W., Daly J., Wilkinson W. E., McCarty K. S., Sr Correlation of estrogen and progesterone receptors with histologic differentiation in mammary carcinoma. Cancer. 1980 Dec 15;46(12 Suppl):2851–2858. doi: 10.1002/1097-0142(19801215)46:12+<2851::aid-cncr2820461424>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  15. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Parl F. F., Schonbaum C. P., Cox D. L., Cavener D. R. Detection of estrogen receptor mRNA in human uterus. Mol Cell Endocrinol. 1987 Aug;52(3):235–242. doi: 10.1016/0303-7207(87)90049-9. [DOI] [PubMed] [Google Scholar]
  17. Reddel R. R., Murphy L. C., Hall R. E., Sutherland R. L. Differential sensitivity of human breast cancer cell lines to the growth-inhibitory effects of tamoxifen. Cancer Res. 1985 Apr;45(4):1525–1531. [PubMed] [Google Scholar]
  18. Rosen P. P., Menendez-Botet C. J., Urban J. A., Fracchia A., Schwartz M. K. Estrogen receptor protein (ERP) in multiple tumor specimens from individual patients with breast cancer. Cancer. 1977 May;39(5):2194–2200. doi: 10.1002/1097-0142(197705)39:5<2194::aid-cncr2820390537>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  19. Simon W. E., Albrecht M., Trams G., Dietel M., Hölzel F. In vitro growth promotion of human mammary carcinoma cells by steroid hormones, tamoxifen, and prolactin. J Natl Cancer Inst. 1984 Aug;73(2):313–321. doi: 10.1093/jnci/73.2.313. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Thorpe S. M. Monoclonal antibody technique for detection of estrogen receptors in human breast cancer: greater sensitivity and more accurate classification of receptor status than the dextran-coated charcoal method. Cancer Res. 1987 Dec 15;47(24 Pt 1):6572–6575. [PubMed] [Google Scholar]
  22. Walter P., Green S., Greene G., Krust A., Bornert J. M., Jeltsch J. M., Staub A., Jensen E., Scrace G., Waterfield M. Cloning of the human estrogen receptor cDNA. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7889–7893. doi: 10.1073/pnas.82.23.7889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Westley B. R., May F. E. Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells. Nucleic Acids Res. 1987 May 11;15(9):3773–3786. doi: 10.1093/nar/15.9.3773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Westley B., May F. E. The human genome contains multiple sequences of varying homology to mouse mammary tumour virus DNA. Gene. 1984 May;28(2):221–227. doi: 10.1016/0378-1119(84)90259-2. [DOI] [PubMed] [Google Scholar]
  25. van Netten J. P., Algard F. T., Coy P., Carlyle S. J., Brigden M. L., Thornton K. R., Peter S., Fraser T., To M. P. Heterogeneous estrogen receptor levels detected via multiple microsamples from individual breast cancers. Cancer. 1985 Oct 15;56(8):2019–2024. doi: 10.1002/1097-0142(19851015)56:8<2019::aid-cncr2820560822>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]

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