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. 1989 Feb;59(2):189–193. doi: 10.1038/bjc.1989.39

Relationship between tumour cell morphology, gap junctions and susceptibility to cytolysis by tumour necrosis factor.

N Matthews 1, M L Neale 1
PMCID: PMC2246987  PMID: 2930684

Abstract

Tumour necrosis factor (TNF) is directly cytolytic to certain tumour cell lines in vitro, although TNF-resistant variants can be selected from these susceptible lines by exposure to TNF. While studying TNF-susceptible L929 cells and their resistant variant, L929/R, we noted that within L929 colonies the cells were widely spaced whereas they were closely packed in L929/R colonies. L929/R cells also adhered more strongly to plastic and differed from L929 in cell shape. Similar observations were made with TNF susceptible and resistant variants of two other cell lines (RK13 and a plastic adherent U937 subline). The tendency of resistant cells to grow closely together suggests the possibility of inter-cell communication for the TNF resistant state. However, like L929 and U937, L929/R and U937/R did not communicate by gap junctions and we could find no evidence of extracellular mediators of TNF resistance. Rather the differences in colonial morphology, cell shape and plastic adherence may be secondary to an underlying mechanism which defines TNF susceptibility/resistance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beutler B., Cerami A. Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316(7):379–385. doi: 10.1056/NEJM198702123160705. [DOI] [PubMed] [Google Scholar]
  2. Darzynkiewicz Z., Williamson B., Carswell E. A., Old L. J. Cell cycle-specific effects of tumor necrosis factor. Cancer Res. 1984 Jan;44(1):83–90. [PubMed] [Google Scholar]
  3. Fletcher W. H., Shiu W. W., Ishida T. A., Haviland D. L., Ware C. F. Resistance to the cytolytic action of lymphotoxin and tumor necrosis factor coincides with the presence of gap junctions uniting target cells. J Immunol. 1987 Aug 1;139(3):956–962. [PubMed] [Google Scholar]
  4. Flick D. A., Gifford G. E. Comparison of in vitro cell cytotoxic assays for tumor necrosis factor. J Immunol Methods. 1984 Mar 30;68(1-2):167–175. doi: 10.1016/0022-1759(84)90147-9. [DOI] [PubMed] [Google Scholar]
  5. Haranaka K., Satomi N., Sakurai A., Haranaka R. Antitumour effects of tumour necrosis factor: cytotoxic or necrotizing activity and its mechanism. Ciba Found Symp. 1987;131:140–153. doi: 10.1002/9780470513521.ch10. [DOI] [PubMed] [Google Scholar]
  6. Kull F. C., Jr, Cuatrecasas P. Possible requirement of internalization in the mechanism of in vitro cytotoxicity in tumor necrosis serum. Cancer Res. 1981 Dec;41(12 Pt 1):4885–4890. [PubMed] [Google Scholar]
  7. Matthews N. Anti-tumour cytotoxin from macrophages: no correlation between cytotoxin adsorption by tumour cell lines and their cytotoxin susceptibility. Immunology. 1984 Nov;53(3):537–543. [PMC free article] [PubMed] [Google Scholar]
  8. Matthews N. Human monocyte cytotoxin is not identical with lymphoblastoid lymphotoxin. Eur J Immunol. 1985 Mar;15(3):311–313. doi: 10.1002/eji.1830150321. [DOI] [PubMed] [Google Scholar]
  9. Matthews N., Watkins J. F. Tumour-necrosis factor from the rabbit. I. Mode of action, specificity and physicochemical properties. Br J Cancer. 1978 Aug;38(2):302–309. doi: 10.1038/bjc.1978.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Neale M. L., Fiera R. A., Matthews N. Involvement of phospholipase A2 activation in tumour cell killing by tumour necrosis factor. Immunology. 1988 May;64(1):81–85. [PMC free article] [PubMed] [Google Scholar]
  11. Ohsawa F., Natori S. Selective degradation of tumor necrosis factor in sensitive cells, and production of membrane-active substance. J Biochem. 1988 Apr;103(4):730–734. doi: 10.1093/oxfordjournals.jbchem.a122336. [DOI] [PubMed] [Google Scholar]
  12. Old L. J. Tumour necrosis factor. Polypeptide mediator network. 1987 Mar 26-Apr 1Nature. 326(6111):330–331. doi: 10.1038/326330a0. [DOI] [PubMed] [Google Scholar]
  13. Rubin B. Y., Anderson S. L., Sullivan S. A., Williamson B. D., Carswell E. A., Old L. J. Nonhematopoietic cells selected for resistance to tumor necrosis factor produce tumor necrosis factor. J Exp Med. 1986 Oct 1;164(4):1350–1355. doi: 10.1084/jem.164.4.1350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ruff M. R., Gifford G. E. Rabbit tumor necrosis factor: mechanism of action. Infect Immun. 1981 Jan;31(1):380–385. doi: 10.1128/iai.31.1.380-385.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Suffys P., Beyaert R., Van Roy F., Fiers W. Reduced tumour necrosis factor-induced cytotoxicity by inhibitors of the arachidonic acid metabolism. Biochem Biophys Res Commun. 1987 Dec 16;149(2):735–743. doi: 10.1016/0006-291x(87)90429-3. [DOI] [PubMed] [Google Scholar]
  16. Taverne J., Matthews N., Depledge P., Playfair J. H. Malarial parasites and tumour cells are killed by the same component of tumour necrosis serum. Clin Exp Immunol. 1984 Aug;57(2):293–300. [PMC free article] [PubMed] [Google Scholar]
  17. el-Fouly M. H., Trosko J. E., Chang C. C. Scrape-loading and dye transfer. A rapid and simple technique to study gap junctional intercellular communication. Exp Cell Res. 1987 Feb;168(2):422–430. doi: 10.1016/0014-4827(87)90014-0. [DOI] [PubMed] [Google Scholar]

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