Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1989 Feb;59(2):217–220. doi: 10.1038/bjc.1989.44

Comparison of the rapid automated MTT-assay with a dye exclusion assay for chemosensitivity testing in childhood leukaemia.

R Pieters 1, D R Huismans 1, A Leyva 1, A J Veerman 1
PMCID: PMC2247012  PMID: 2930687

Full text

PDF
217

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ajani J. A., Baker F. L., Spitzer G., Kelly A., Brock W., Tomasovic B., Singletary S. E., McMurtrey M., Plager C. Comparison between clinical response and in vitro drug sensitivity of primary human tumors in the adhesive tumor cell culture system. J Clin Oncol. 1987 Dec;5(12):1912–1921. doi: 10.1200/JCO.1987.5.12.1912. [DOI] [PubMed] [Google Scholar]
  2. Alley M. C., Scudiero D. A., Monks A., Hursey M. L., Czerwinski M. J., Fine D. L., Abbott B. J., Mayo J. G., Shoemaker R. H., Boyd M. R. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 1988 Feb 1;48(3):589–601. [PubMed] [Google Scholar]
  3. Bird M. C., Bosanquet A. G., Forskitt S., Gilby E. D. Long-term comparison of results of a drug sensitivity assay in vitro with patient response in lymphatic neoplasms. Cancer. 1988 Mar 15;61(6):1104–1109. doi: 10.1002/1097-0142(19880315)61:6<1104::aid-cncr2820610609>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  4. Bird M. C., Bosanquet A. G., Forskitt S., Gilby E. D. Semi-micro adaptation of a 4-day differential staining cytotoxicity (DiSC) assay for determining the in-vitro chemosensitivity of haematological malignancies. Leuk Res. 1986;10(4):445–449. doi: 10.1016/0145-2126(86)90075-5. [DOI] [PubMed] [Google Scholar]
  5. Bird M. C., Godwin V. A., Antrobus J. H., Bosanquet A. G. Comparison of in vitro drug sensitivity by the differential staining cytotoxicity (DiSC) and colony-forming assays. Br J Cancer. 1987 Apr;55(4):429–431. doi: 10.1038/bjc.1987.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bosanquet A. G., Bird M. C., Price W. J., Gilby E. D. An assessment of a short-term tumour chemosensitivity assay in chronic lymphocytic leukaemia. Br J Cancer. 1983 Jun;47(6):781–789. doi: 10.1038/bjc.1983.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carmichael J., DeGraff W. G., Gazdar A. F., Minna J. D., Mitchell J. B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987 Feb 15;47(4):936–942. [PubMed] [Google Scholar]
  8. Cole S. P. Rapid chemosensitivity testing of human lung tumor cells using the MTT assay. Cancer Chemother Pharmacol. 1986;17(3):259–263. doi: 10.1007/BF00256695. [DOI] [PubMed] [Google Scholar]
  9. Finlay G. J., Wilson W. R., Baguley B. C. Comparison of in vitro activity of cytotoxic drugs towards human carcinoma and leukaemia cell lines. Eur J Cancer Clin Oncol. 1986 Jun;22(6):655–662. doi: 10.1016/0277-5379(86)90162-8. [DOI] [PubMed] [Google Scholar]
  10. Hongo T., Fujii Y., Mizuno Y., Haraguchi S., Yoshida T. O. [Anticancer drug sensitivity test using the short-term microplate culture and MTT dye reduction assay]. Gan To Kagaku Ryoho. 1987 Feb;14(2):472–478. [PubMed] [Google Scholar]
  11. Laurent G., Kuhlein E., Casellas P., Canat X., Carayon P., Poncelet P., Correll S., Rigal F., Jansen F. K. Determination of sensitivity of fresh leukemia cells to immunotoxins. Cancer Res. 1986 May;46(5):2289–2294. [PubMed] [Google Scholar]
  12. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55–63. doi: 10.1016/0022-1759(83)90303-4. [DOI] [PubMed] [Google Scholar]
  13. Park J. G., Kramer B. S., Steinberg S. M., Carmichael J., Collins J. M., Minna J. D., Gazdar A. F. Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay. Cancer Res. 1987 Nov 15;47(22):5875–5879. [PubMed] [Google Scholar]
  14. Pieters R., Huismans D. R., Leyva A., Veerman A. J. Adaptation of the rapid automated tetrazolium dye based (MTT) assay for chemosensitivity testing in childhood leukemia. Cancer Lett. 1988 Aug 30;41(3):323–332. doi: 10.1016/0304-3835(88)90294-7. [DOI] [PubMed] [Google Scholar]
  15. Salem M., Delwel R., Touw I., Mahmoud L., Löwenberg B. Human AML colony growth in serum-free culture. Leuk Res. 1988;12(2):157–165. doi: 10.1016/0145-2126(88)90076-8. [DOI] [PubMed] [Google Scholar]
  16. Twentyman P. R., Luscombe M. A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br J Cancer. 1987 Sep;56(3):279–285. doi: 10.1038/bjc.1987.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weisenthal L. M., Dill P. L., Kurnick N. B., Lippman M. E. Comparison of dye exclusion assays with a clonogenic assay in the determination of drug-induced cytotoxicity. Cancer Res. 1983 Jan;43(1):258–264. [PubMed] [Google Scholar]
  18. Weisenthal L. M., Lippman M. E. Clonogenic and nonclonogenic in vitro chemosensitivity assays. Cancer Treat Rep. 1985 Jun;69(6):615–632. [PubMed] [Google Scholar]
  19. Weisenthal L. M., Marsden J. A., Dill P. L., Macaluso C. K. A novel dye exclusion method for testing in vitro chemosensitivity of human tumors. Cancer Res. 1983 Feb;43(2):749–757. [PubMed] [Google Scholar]
  20. Weisenthal L. M., Su Y. Z., Duarte T. E., Dill P. L., Nagourney R. A. Perturbation of in vitro drug resistance in human lymphatic neoplasms by combinations of putative inhibitors of protein kinase C. Cancer Treat Rep. 1987 Dec;71(12):1239–1243. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES