Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1989 Apr;59(4):569–572. doi: 10.1038/bjc.1989.115

Effects of chemotherapeutic agents on alpha-fetoprotein secretion and growth of human hepatoma cell lines in vitro.

A Muraoka 1, T Tokiwa 1, J Sato 1
PMCID: PMC2247148  PMID: 2469455

Abstract

The effects of various chemotherapeutic agents on alpha-fetoprotein (AFP) secretion and growth of human hepatocellular carcinoma and hepatoblastoma cell lines were investigated in vitro. It was found that there was a high correlation between hepatoma cell number and AFP secretion after treatment and that the amount of AFP secreted per cell per 72 h was not affected with therapeutically achievable concentrations. These results suggest that serum AFP level in patients with hepatomas does not correlate with the size of whole tumour but with that of viable tumour mass, and that AFP-secreting capacity of tumour cells in the mass is kept unchanged after chemotherapy.

Full text

PDF
569

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassendine M. F., Wright N. A., Thomas H. C., Sherlock S. Growth characteristics of alpha-foetoprotein-secreting human hepatocellular carcinoma in athymic (nude) mice. Clin Sci (Lond) 1983 Jun;64(6):643–648. doi: 10.1042/cs0640643. [DOI] [PubMed] [Google Scholar]
  2. Curtin N. J., Harris A. L., James O. F., Bassendine M. F. Inhibition of the growth of human hepatocellular carcinoma in vitro and in athymic mice by a quinazoline inhibitor of thymidylate synthase, CB3717. Br J Cancer. 1986 Mar;53(3):361–368. doi: 10.1038/bjc.1986.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doi I. Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma. Gan. 1976 Feb;67(1):1–10. [PubMed] [Google Scholar]
  4. Johnson P. J., Williams R. Serum alpha-fetoprotein estimations and doubling time in hepatocellular carcinoma: influence of therapy and possible value in early detection. J Natl Cancer Inst. 1980 Jun;64(6):1329–1332. doi: 10.1093/jnci/64.6.1329. [DOI] [PubMed] [Google Scholar]
  5. Johnson P. J., Williams R., Thomas H., Sherlock S., Murray-Lyon I. M. Induction of remission in hepatocellular carcinoma with doxorubicin. Lancet. 1978 May 13;1(8072):1006–1009. doi: 10.1016/s0140-6736(78)90735-3. [DOI] [PubMed] [Google Scholar]
  6. Lo K. W., Miller E. E., Morris H. P., Tsou K. C. Chemotherapy of Morris hepatoma 3924A: correlation of size and weight of tumor and preliminary data with 5-fluoro-2'-deoxyuridine (5-FUDR; NSC-27640). Cancer Chemother Rep. 1973 Sep-Oct;57(3):245–249. [PubMed] [Google Scholar]
  7. Matsumoto Y., Suzuki T., Ono H., Nakase A., Honjo I. Response of alpha-fetoprotein to chemotherapy in patients with hepatomas. Cancer. 1974 Nov;34(5):1602–1606. doi: 10.1002/1097-0142(197411)34:5<1602::aid-cncr2820340507>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  8. McIntire K. R., Vogel C. L., Primack A., Waldmann T. A., Kyalwazi S. K. Effect of surgical and chemotherapeutic treatment on alpha-fetoprotein levels in patients with hepatocellular carcinoma. Cancer. 1976 Feb;37(2):677–683. doi: 10.1002/1097-0142(197602)37:2<677::aid-cncr2820370211>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  9. Nakabayashi H., Taketa K., Miyano K., Yamane T., Sato J. Growth of human hepatoma cells lines with differentiated functions in chemically defined medium. Cancer Res. 1982 Sep;42(9):3858–3863. [PubMed] [Google Scholar]
  10. Pritchard J., da Cunha A., Cornbleet M. A., Carter C. J. Alpha feta (alpha FP) monitoring of response to adriamycin in hepatoblastoma. J Pediatr Surg. 1982 Aug;17(4):429–430. doi: 10.1016/s0022-3468(82)80508-3. [DOI] [PubMed] [Google Scholar]
  11. Purves L. R., Bersohn I., Geddes E. W. Serum alpha-feto-protein and primary cancer of the liver in man. Cancer. 1970 Jun;25(6):1261–1270. doi: 10.1002/1097-0142(197006)25:6<1261::aid-cncr2820250603>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  12. Raghavan D., Gibbs J., Nogueira Costa R., Kohn J., Orr A. H., Barrett A., Peckham M. J. The interpretation of marker protein assays: a critical appraisal in clinical studies and a xenograft model. Br J Cancer Suppl. 1980 Apr;4:191–194. [PMC free article] [PubMed] [Google Scholar]
  13. Salmon S. E. Human tumor colony assay and chemosensitivity testing. Cancer Treat Rep. 1984 Jan;68(1):117–125. [PubMed] [Google Scholar]
  14. Sell S., Wepsic H. T., Nickel R., Nichols M. Rat alpha1 fetoprotein. IV. Effect of growth and surgical removal of Morris hepatoma 7777 on the serum alpha1F concentration of Buffalo rats. J Natl Cancer Inst. 1974 Jan;52(1):133–137. doi: 10.1093/jnci/52.1.133. [DOI] [PubMed] [Google Scholar]
  15. Slee P. H., de Bruijn E. A., Leeflang P., Kuppen P. J., van den Berg L., van Oosterom A. T. Variations in exposure to mitomycin C in an in vitro colony-forming assay. Br J Cancer. 1986 Dec;54(6):951–955. doi: 10.1038/bjc.1986.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tokiwa T., Miyagiwa M., Kusaka Y., Muraoka A., Sato J. Effects of various substrates on human hepatoblastoma and hepatoma cell culture. Cell Biol Int Rep. 1988 Feb;12(2):131–142. doi: 10.1016/0309-1651(88)90127-0. [DOI] [PubMed] [Google Scholar]
  17. Tsukada Y., Mikuni M., Hirai H. In vitro cloning of a rat ascites hepatoma cell line, AH66, with special reference to alpha-fetoprotein synthesis. Int J Cancer. 1974 Feb 15;13(2):196–202. doi: 10.1002/ijc.2910130207. [DOI] [PubMed] [Google Scholar]
  18. Von Hoff D. D., Casper J., Bradley E., Sandbach J., Jones D., Makuch R. Association between human tumor colony-forming assay results and response of an individual patient's tumor to chemotherapy. Am J Med. 1981 May;70(5):1027–1041. doi: 10.1016/0002-9343(81)90859-7. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES