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. 1989 Dec;60(6):897–903. doi: 10.1038/bjc.1989.387

Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.

N R Lemoine 1, E S Mayall 1, T Jones 1, D Sheer 1, S McDermid 1, P Kendall-Taylor 1, D Wynford-Thomas 1
PMCID: PMC2247263  PMID: 2557880

Abstract

Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.

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Selected References

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