Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1989 Jul;60(1):41–44. doi: 10.1038/bjc.1989.216

Epidermal growth factor receptors in human prostate cancer: correlation with histological differentiation of the tumour.

S Q Maddy 1, G D Chisholm 1, A Busuttil 1, F K Habib 1
PMCID: PMC2247342  PMID: 2478180

Abstract

The presence of specific and high affinity epidermal growth factor receptors (EGF-R) has been demonstrated in human prostate cancer (CaP). Scatchard analysis of the binding data revealed a linear plot consistent with a single class of binding sites with a mean dissociation constant (Kd) +/- s.d. = 1.6 +/- 0.4 nmol 1-1. Additionally the binding was specific for EGF since no other competitor than EGF was able to displace the binding of the labelled ligand from its receptor. Comparison of the concentrations of EGF-R in tissues from 19 patients with CaP with those measured in a group of 18 patients with benign prostatic hyperplasia (BPH) reveal that the expression of EGF-R was significantly higher in BPH (mean +/- s.d. = 125 +/- 7 fmol mg protein-1) than in CaP (52 +/- 11 fmol mg protein-1; P less than 0.01). Furthermore, in CaP the expression of EGF-R varied according to the histological grade of the cancer: well differentiated tumours demonstrated more receptors (84 +/- 13 fmol mg protein-1) than poorly differentiated tumours (22 +/- 5 fmol mg protein-1; P less than 0.01). Clearly the depletion in the expression of EGF receptors in CaP is a function of the histological grade of the cancer and as such EGF receptors could be used as a biochemical marker for tumour differentiation.

Full text

PDF
41

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banks-Schlegel S. P., Quintero J. Human esophageal carcinoma cells have fewer, but higher affinity epidermal growth factor receptors. J Biol Chem. 1986 Apr 5;261(10):4359–4362. [PubMed] [Google Scholar]
  2. Beynon L. L., Busuttil A., Newsam J. E., Chisholm G. D. Incidental carcinoma of the prostate: selection for deferred treatment. Br J Urol. 1983 Dec;55(6):733–736. doi: 10.1111/j.1464-410x.1983.tb03415.x. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Bradley S. J., Garfinkle G., Walker E., Salem R., Chen L. B., Steele G., Jr Increased expression of the epidermal growth factor receptor on human colon carcinoma cells. Arch Surg. 1986 Nov;121(11):1242–1247. doi: 10.1001/archsurg.121.11.1242. [DOI] [PubMed] [Google Scholar]
  5. Carpenter G., Cohen S. Epidermal growth factor. Annu Rev Biochem. 1979;48:193–216. doi: 10.1146/annurev.bi.48.070179.001205. [DOI] [PubMed] [Google Scholar]
  6. Chaproniere D. M., McKeehan W. L. Serial culture of single adult human prostatic epithelial cells in serum-free medium containing low calcium and a new growth factor from bovine brain. Cancer Res. 1986 Feb;46(2):819–824. [PubMed] [Google Scholar]
  7. Cowley G. P., Smith J. A., Gusterson B. A. Increased EGF receptors on human squamous carcinoma cell lines. Br J Cancer. 1986 Feb;53(2):223–229. doi: 10.1038/bjc.1986.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Downward J., Yarden Y., Mayes E., Scrace G., Totty N., Stockwell P., Ullrich A., Schlessinger J., Waterfield M. D. Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences. Nature. 1984 Feb 9;307(5951):521–527. doi: 10.1038/307521a0. [DOI] [PubMed] [Google Scholar]
  9. Eaton C. L., Davies P., Phillips M. E. Growth factor involvement and oncogene expression in prostatic tumours. J Steroid Biochem. 1988;30(1-6):341–345. doi: 10.1016/0022-4731(88)90120-3. [DOI] [PubMed] [Google Scholar]
  10. Edery M., Pang K., Larson L., Colosi T., Nandi S. Epidermal growth factor receptor levels in mouse mammary glands in various physiological states. Endocrinology. 1985 Jul;117(1):405–411. doi: 10.1210/endo-117-1-405. [DOI] [PubMed] [Google Scholar]
  11. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  12. Gullick W. J., Marsden J. J., Whittle N., Ward B., Bobrow L., Waterfield M. D. Expression of epidermal growth factor receptors on human cervical, ovarian, and vulval carcinomas. Cancer Res. 1986 Jan;46(1):285–292. [PubMed] [Google Scholar]
  13. Gusterson B., Cowley G., Smith J. A., Ozanne B. Cellular localisation of human epidermal growth factor receptor. Cell Biol Int Rep. 1984 Aug;8(8):649–658. doi: 10.1016/0309-1651(84)90045-6. [DOI] [PubMed] [Google Scholar]
  14. Hwang D. L., Tay Y. C., Lin S. S., Lev-Ran A. Expression of epidermal growth factor receptors in human lung tumors. Cancer. 1986 Nov 15;58(10):2260–2263. doi: 10.1002/1097-0142(19861115)58:10<2260::aid-cncr2820581017>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  15. Kay D. G., Lai W. H., Uchihashi M., Khan M. N., Posner B. I., Bergeron J. J. Epidermal growth factor receptor kinase translocation and activation in vivo. J Biol Chem. 1986 Jun 25;261(18):8473–8480. [PubMed] [Google Scholar]
  16. Maddy S. Q., Chisholm G. D., Hawkins R. A., Habib F. K. Localization of epidermal growth factor receptors in the human prostate by biochemical and immunocytochemical methods. J Endocrinol. 1987 Apr;113(1):147–153. doi: 10.1677/joe.0.1130147. [DOI] [PubMed] [Google Scholar]
  17. Neal D. E., Marsh C., Bennett M. K., Abel P. D., Hall R. R., Sainsbury J. R., Harris A. L. Epidermal-growth-factor receptors in human bladder cancer: comparison of invasive and superficial tumours. Lancet. 1985 Feb 16;1(8425):366–368. doi: 10.1016/s0140-6736(85)91386-8. [DOI] [PubMed] [Google Scholar]
  18. Pontes J. E., Chu T. M., Slack N., Karr J., Murphy G. P. Serum prostatic antigen measurement in localized prostatic cancer: correlation with clinical course. J Urol. 1982 Dec;128(6):1216–1218. doi: 10.1016/s0022-5347(17)53431-x. [DOI] [PubMed] [Google Scholar]
  19. Robinson R. A., Branum E. L., Volkenant M. E., Moses H. L. Cell cycle variation in 125I-labeled epidermal growth factor binding in chemically transformed cells. Cancer Res. 1982 Jul;42(7):2633–2638. [PubMed] [Google Scholar]
  20. Sporn M. B., Roberts A. B. Peptide growth factors are multifunctional. Nature. 1988 Mar 17;332(6161):217–219. doi: 10.1038/332217a0. [DOI] [PubMed] [Google Scholar]
  21. de Larco J. E., Todaro G. J. Growth factors from murine sarcoma virus-transformed cells. Proc Natl Acad Sci U S A. 1978 Aug;75(8):4001–4005. doi: 10.1073/pnas.75.8.4001. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES