Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Jul;135(1):39–44. doi: 10.1128/jb.135.1.39-44.1978

Kinetics of glucose repression of yeast cytochrome c.

R S Zitomer, D L Nichols
PMCID: PMC224761  PMID: 209012

Abstract

The kinetics of glucose repression of cytochrome c synthesis was measured by a radioimmune assay. When 5 or 10% glucose was added to a derepressed culture, the rate of cytochrome c synthesis was reduced to the repressed level with a half-life of 2 min. The addition of 1 or 0.5% glucose repressed the rate of cytochrome c synthesis to the same level as high glucose concentrations but with a longer half-life of 3 min. Glucose repression had no effect on the stability or function of the cytochrome c protein. Cellular levels of active cytochrome c mRNA during glucose repression were measured by translation of total cellular polyadenylic acid-containing RNA and immunoprecipitation cytochrome c from the translation products. The results of these measurements indicate that glucose represses the rate of cytochrome c synthesis through a reduction in the level of translatable cytochrome c mRNA.

Full text

PDF
39

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bogorad L. Evolution of organelles and eukaryotic genomes. Science. 1975 May 30;188(4191):891–898. doi: 10.1126/science.1138359. [DOI] [PubMed] [Google Scholar]
  2. Borst P. Mitochondrial nucleic acids. Annu Rev Biochem. 1972;41:333–376. doi: 10.1146/annurev.bi.41.070172.002001. [DOI] [PubMed] [Google Scholar]
  3. Bossinger J., Cooper T. G. Molecular events associated with induction of arginase in Saccharomyces cerevisiae. J Bacteriol. 1977 Jul;131(1):163–173. doi: 10.1128/jb.131.1.163-173.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bossinger J., Cooper T. G. Sequence of molecular events involved in induction of allophanate hydrolase. J Bacteriol. 1976 Apr;126(1):198–204. doi: 10.1128/jb.126.1.198-204.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Butow R. A., Ferguson M. J., Cederbaum A. Low-temperature induction of respiratory deficiency in yeast mutants. Biochemistry. 1973 Jan 2;12(1):158–164. doi: 10.1021/bi00725a026. [DOI] [PubMed] [Google Scholar]
  6. Creanor J., May J. W., Mitchison J. M. The effect of 8-hydroxyquinoline on enzyme synthesis in the fission yeast Schizosaccharomyces pombe. Eur J Biochem. 1975 Dec 15;60(2):487–493. doi: 10.1111/j.1432-1033.1975.tb21027.x. [DOI] [PubMed] [Google Scholar]
  7. Ebner E., Mennucci L., Schatz G. Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. I. Effect of nuclear mutations on mitochondrial protein synthesis. J Biol Chem. 1973 Aug 10;248(15):5360–5368. [PubMed] [Google Scholar]
  8. Fraser R. S. Turnover of polyadenylated messenger RNA in fission yeast. Evidence for the control of protein synthesis at the translational level. Eur J Biochem. 1975 Dec 15;60(2):477–486. doi: 10.1111/j.1432-1033.1975.tb21026.x. [DOI] [PubMed] [Google Scholar]
  9. Henson C. P., Perlman P., Weber C. N., Mahler H. R. Formation of yeast mitochondria. II. Effects of antibiotics on enzyme activity during derepression. Biochemistry. 1968 Dec;7(12):4445–4454. doi: 10.1021/bi00852a041. [DOI] [PubMed] [Google Scholar]
  10. Hynes N. E., Phillips S. L. Turnover of polyadenylate-containing ribonucleic acid in Saccharomyces cerevisiae. J Bacteriol. 1976 Feb;125(2):595–600. doi: 10.1128/jb.125.2.595-600.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jayaraman J., Cotman C., Mahler H. R., Sharp C. W. Biochemical correlates of respiratory deficiency. VII. Glucose repression. Arch Biochem Biophys. 1966 Sep 26;116(1):224–251. doi: 10.1016/0003-9861(66)90029-4. [DOI] [PubMed] [Google Scholar]
  12. Lawther R. P., Cooper T. G. Effects of inducer addition and removal upon the level of allophanate hydrolase in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1973 Dec 19;55(4):1100–1104. doi: 10.1016/s0006-291x(73)80008-7. [DOI] [PubMed] [Google Scholar]
  13. Lawther R. P., Cooper T. G. Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae. J Bacteriol. 1975 Mar;121(3):1064–1073. doi: 10.1128/jb.121.3.1064-1073.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Linnane A. W., Haslam J. M., Lukins H. B., Nagley P. The biogenesis of mitochondria in microorganisms. Annu Rev Microbiol. 1972;26:163–198. doi: 10.1146/annurev.mi.26.100172.001115. [DOI] [PubMed] [Google Scholar]
  15. Mahler H. R. Biogenetic autonomy of mitochondria. CRC Crit Rev Biochem. 1973 Aug;1(3):381–460. doi: 10.3109/10409237309105439. [DOI] [PubMed] [Google Scholar]
  16. Messenguy F., Cooper T. G. Evidence that specific and "general" control of ornithine carbamoyltransferase production occurs at the level of transcription in Saccharomyces cerevisiae. J Bacteriol. 1977 Jun;130(3):1253–1261. doi: 10.1128/jb.130.3.1253-1261.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perlman P. S., Mahler H. R. Derepression of mitochondria and their enzymes in yeast: regulatory aspects. Arch Biochem Biophys. 1974 May;162(1):248–271. doi: 10.1016/0003-9861(74)90125-8. [DOI] [PubMed] [Google Scholar]
  18. Petersen N. S., McLaughlin C. S., Nierlich D. P. Half life of yeast messenger RNA. Nature. 1976 Mar 4;260(5546):70–72. doi: 10.1038/260070a0. [DOI] [PubMed] [Google Scholar]
  19. REILLY C., SHERMAN R. GLUCOSE REPRESSION OF CYTOCHROME A-SYNTHESIS IN CYTOCHROME-DEFICIENT MUTANTS OF YEAST. Biochim Biophys Acta. 1965 Apr 19;95:640–651. doi: 10.1016/0005-2787(65)90518-6. [DOI] [PubMed] [Google Scholar]
  20. Rouslin W., Schatz G. Interdependence between promitochondrial and cytoplasmic protein synthesis during respiratory adaptation in baker's yeast. Biochem Biophys Res Commun. 1969 Dec 4;37(6):1002–1007. doi: 10.1016/0006-291x(69)90231-9. [DOI] [PubMed] [Google Scholar]
  21. Sherman F., Stewart J. W. Genetics and biosynthesis of cytochrome c. Annu Rev Genet. 1971;5:257–296. doi: 10.1146/annurev.ge.05.120171.001353. [DOI] [PubMed] [Google Scholar]
  22. Sherman F., Stewart J. W., Jackson M., Gilmore R. A., Parker J. H. Mutants of yeast defective in iso-1-cytochrome c. Genetics. 1974 Jun;77(2):255–284. doi: 10.1093/genetics/77.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sherman F., Stewart J. W., Margoliash E., Parker J., Campbell W. The structural gene for yeast cytochrome C. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1498–1504. doi: 10.1073/pnas.55.6.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Simchen G., Salts Y., Piñon R. Sensitivity of meiotic yeast cells to ultraviolet light. Genetics. 1973 Apr;73(4):531–541. doi: 10.1093/genetics/73.4.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tonnesen T., Friesen J. D. Inhibitors of ribonucleic acid synthesis in Saccharomyces cerevisiae: decay rate of messenger ribonucleic acid. J Bacteriol. 1973 Sep;115(3):889–896. doi: 10.1128/jb.115.3.889-896.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES