Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1968 Apr;59(4):1144–1151. doi: 10.1073/pnas.59.4.1144

The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes.

J Abbott, H Holtzer
PMCID: PMC224844  PMID: 5240020

Full text

PDF
1144

Images in this article

1146Image
on p.1146
1146Image
on p.1146
1147Image
on p.1147

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott J., Holtzer H. The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures. J Cell Biol. 1966 Mar;28(3):473–487. doi: 10.1083/jcb.28.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COMAN D. R. Adhesiveness and stickiness: two independent properties of the cell surface. Cancer Res. 1961 Nov;21:1436–1438. [PubMed] [Google Scholar]
  3. Coon H. G., Cahn R. D. Differentiation in vitro: effects of Sephadex fractions of chick embryo extract. Science. 1966 Sep 2;153(3740):1116–1119. doi: 10.1126/science.153.3740.1116. [DOI] [PubMed] [Google Scholar]
  4. Coon H. G. Clonal stability and phenotypic expression of chick cartilage cells in vitro. Proc Natl Acad Sci U S A. 1966 Jan;55(1):66–73. doi: 10.1073/pnas.55.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DJORDJEVIC B., SZYBALSKI W. Genetics of human cell lines. III. Incorporation of 5-bromo- and 5-iododeoxyuridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity. J Exp Med. 1960 Sep 1;112:509–531. doi: 10.1084/jem.112.3.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DUTTON R. W., DUTTON A. H., VAUGHAN J. H. The effect of 5-bromouracil deoxyriboside on the synthesis of antibody in vitro. Biochem J. 1960 May;75:230–235. doi: 10.1042/bj0750230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. EIDINOFF M. L., CHEONG L., RICH M. A. Incorporation of unnatural pyrimidine bases into deoxyribonucleic acid of mammalian cells. Science. 1959 Jun 5;129(3362):1550–1551. doi: 10.1126/science.129.3362.1550. [DOI] [PubMed] [Google Scholar]
  8. HAKALA M. T. Effect of 5-bromodeoxyuridine incorporation on survival of cultured mammalian cells. Biochim Biophys Acta. 1962 Nov 26;61:815–823. doi: 10.1016/0926-6550(62)90064-6. [DOI] [PubMed] [Google Scholar]
  9. HAKALA M. T. Mode of action of 5-bromodeoxyuridine on mammalian cells in culture. J Biol Chem. 1959 Dec;234:3072–3076. [PubMed] [Google Scholar]
  10. HAM R. G. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM. Proc Natl Acad Sci U S A. 1965 Feb;53:288–293. doi: 10.1073/pnas.53.2.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holtzer H., Abbott J., Lash J., Holtzer S. THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS IN VITRO, I. DEDIFFERENTIATION OF CARTILAGE CELLS. Proc Natl Acad Sci U S A. 1960 Dec;46(12):1533–1542. doi: 10.1073/pnas.46.12.1533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KAJIWARA K., MUELLER G. C. MOLECULAR EVENTS IN THE REPRODUCTION OF ANIMAL CELLS. 3. FRACTIONAL SYNTHESIS OF DEOXYRIBONUCLEIC ACID WITH 5-BROMODEOXYURIDINE AND ITS EFFECT ON CLONING EFFICIENCY. Biochim Biophys Acta. 1964 Nov 15;91:486–493. [PubMed] [Google Scholar]
  13. KURODA Y. STUDIES ON CARTILAGE CELLS IN VITRO. I. MORPHOLOGY AND GROWTH OF CARTILAGE CELLS IN MONOLAYER CULTURES. Exp Cell Res. 1964 Jul;35:326–336. doi: 10.1016/0014-4827(64)90099-0. [DOI] [PubMed] [Google Scholar]
  14. LITTLEFIELD J. W., GOULD E. A. The toxic effect of 5-bromodeoxyuridine on cultured epithelial cells. J Biol Chem. 1960 Apr;235:1129–1133. [PubMed] [Google Scholar]
  15. Morris N. R., Cramer J. W. DNA synthesis by mammalian cells inhibited in culture by 5-lodo-2'-deoxyuridine. Mol Pharmacol. 1966 Jan;2(1):1–9. [PubMed] [Google Scholar]
  16. Nameroff M., Holtzer H. The loss of phenotypic traits by differentiated cells. IV. Changes in polysaccharides produced by dividing chondrocytes. Dev Biol. 1967 Sep;16(3):250–281. doi: 10.1016/0012-1606(67)90026-7. [DOI] [PubMed] [Google Scholar]
  17. Okazaki K., Holtzer H. An analysis of myogenesis in vitro using fluorescein-labeled antimyosin. J Histochem Cytochem. 1965 Nov-Dec;13(8):726–739. doi: 10.1177/13.8.726. [DOI] [PubMed] [Google Scholar]
  18. Puck T. T., Kao F. T. Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1227–1234. doi: 10.1073/pnas.58.3.1227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. STOCKDALE F., OKAZAKI K., NAMEROFF M., HOLTZER H. 5-BROMODEOXYURIDINE: EFFECT ON MYOGENESIS IN VITRO. Science. 1964 Oct 23;146(3643):533–535. doi: 10.1126/science.146.3643.533. [DOI] [PubMed] [Google Scholar]
  20. WESSELLS N. K. DNA SYNTHESIS, MITOSIS, AND DIFFERENTIATION IN PANCREATIC ACINAR CELLS IN VITRO. J Cell Biol. 1964 Mar;20:415–433. doi: 10.1083/jcb.20.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WILT F. H. REGULATION OF THE INITIATION OF CHICK EMBRYO HEMOGLOBIN SYNTHESIS. J Mol Biol. 1965 Jun;12:331–341. doi: 10.1016/s0022-2836(65)80257-1. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES