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. 1987 Aug;99(1):179–189. doi: 10.1017/s0950268800067017

Haemophilus influenzae subtyping by SDS-PAGE of whole-cell polypeptides.

A J Paterson, K F Macsween, T H Pennington
PMCID: PMC2249172  PMID: 3497048

Abstract

Strains of Haemophilus influenzae isolated from patients in N.E. Scotland between 1983 and 1986 have been subtyped by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell polypeptides. Gels were stained with Coomassie blue and polypeptide profiles were analysed using the Dice coefficient of similarity. Type b strains were all closely related, the 19 strains analysed being grouped at a 90% similarity level into one large (13 strains) and one small (3 strains) cluster with 3 strains being ungrouped. Thirty-six non-typable, epidemiologically unrelated strains were subtyped; one pair of strains had indistinguishable polypeptide profiles. The polypeptide profiles of the remaining strains showed much heterogeneity, although groups of strains isolated from the same patient over short periods showed indistinguishable profiles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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