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. 1987 Aug;99(1):191–200. doi: 10.1017/s0950268800067029

Characterization of non-typable strains of Staphylococcus aureus from cases of hospital infection.

A Vindel, C Martín-Bourgon, J A Saez-Nieto
PMCID: PMC2249189  PMID: 3609172

Abstract

A high percentage of non-typable (NT) Staphylococcus aureus strains was isolated in Spanish hospitals during 1984 and 1985. Several alternative methods of typing were employed to study these isolates. These were: phage-typing at 1000 X RTD, phage-typing after heat-treatment (48 degrees C), thermal shock (56 degrees C), reverse-typing and induction of additional phages. Using these methods the number of NT isolates was reduced by 60%. Best results were obtained with heat-treatment. Additional phages and reverse-typing were also useful. A scheme for the study of outbreaks and sporadic cases caused by NT strains is proposed using the methods described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry A. L., Lachica R. V., Atchison F. W. Identification of Staphylococcus aureus by simultaneous use of tube coagulase and thermonuclease tests. Appl Microbiol. 1973 Mar;25(3):496–497. doi: 10.1128/am.25.3.496-497.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dowsett E. G., Petts D. N., Baker S. L., DeSaxe M. J., Coe A. E., Naidoo J., Noble W. C. Analysis of an outbreak of staphylococcal scalded skin syndrome: strategies for typing 'non-typable' strains. J Hosp Infect. 1984 Dec;5(4):391–397. doi: 10.1016/0195-6701(84)90007-0. [DOI] [PubMed] [Google Scholar]
  3. Espersen F., Nielsen P. B., Lund K., Sylvest B., Jensen K. Hospital-acquired infections in a burns unit caused by an imported strain of Staphylococcus aureus with unusual multi-resistance. J Hyg (Lond) 1982 Jun;88(3):535–541. doi: 10.1017/s002217240007039x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lacey R. W., Keyworth N., Lincoln C. Staphylococci in the U.K.: a review. J Antimicrob Chemother. 1984 Dec;14 (Suppl 500):19–25. doi: 10.1093/jac/14.suppl_d.19. [DOI] [PubMed] [Google Scholar]
  5. Linnemann C. C., Jr, Mason M., Moore P., Korfhagen T. R., Staneck J. L. Methicillin-resistant Staphylococcus aureus: experience in a general hospital over four years. Am J Epidemiol. 1982 Jun;115(6):941–950. doi: 10.1093/oxfordjournals.aje.a113381. [DOI] [PubMed] [Google Scholar]
  6. Lorian V., Zak O., Suter J., Bruecher C. Staphylococci, in vitro and in vivo. Diagn Microbiol Infect Dis. 1985 Sep;3(5):433–444. doi: 10.1016/0732-8893(85)90082-3. [DOI] [PubMed] [Google Scholar]
  7. Martín-Bourgon C., Berrón S., Casal J. Hospital infection caused by non-typable Staphylococcus aureus: application of reverse typing. J Hyg (Lond) 1985 Apr;94(2):201–204. doi: 10.1017/s0022172400061404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Martín-Bourgon C. Phage-typing of Staphylococcus aureus from cases of bacteraemia. J Hosp Infect. 1985 Dec;6(4):429–433. doi: 10.1016/0195-6701(85)90061-1. [DOI] [PubMed] [Google Scholar]
  9. Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol. 1985 Nov;22(5):828–834. doi: 10.1128/jcm.22.5.828-834.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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