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. 1987 Dec;99(3):745–753. doi: 10.1017/s0950268800066607

Egg-grown and tissue-culture-grown variants of influenza A (H3N2) virus with special attention to their use as antigens in seroepidemiology.

R Pyhälä 1, L Pyhälä 1, M Valle 1, K Aho 1
PMCID: PMC2249244  PMID: 3428377

Abstract

A field strain of influenza A (H3N2) virus isolated in embryonated eggs during the 1984-5 influenza outbreak (A/Finland/13/85E) was compared in an antigenic analysis with virus from the same clinical specimen isolated in MDCK cell cultures (A/Finland/13/85M). The M-virus appeared to be more sensitive to haemagglutination-inhibiting antibodies against heterologous viruses than did the E-virus. The results of propagation and plaque purification experiments support the hypothesis that a single clinical specimen may consist of distinct antigenic variant subpopulations promoted selectively by the host during isolation procedures. Receptor-binding properties are discussed as a possible explanation for this selectivity. A set of 471 paired sera consisting of pre-epidemic and post-epidemic specimens taken from the same subjects in 1984-5 was studied for haemagglutination-inhibiting antibodies to six influenza A (H3N2) virus strains, including the E-virus and the M-virus from A/Finland/13/85. Of the antigens used, the M-virus detected significant antibody increases more frequently than did the E-virus (10.0 v. 5.9%). The superiority of the M-virus may rest primarily in its ability to pick out anamnestic antibody responses. Irrespective of this cross-reactivity, pre-epidemic antibody to the M-virus was fairly well associated with protection. In the set of sera (230 specimens) collected in summer 1985 to represent different age groups, the antibody status against the M-virus was significantly better than the status against the E-virus. The results suggest that, at least in some instances, antibody to MDCK-grown virus is a more accurate indicator of the immune status of a community than antibodies to egg-grown virus variants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beyer W. E., Masurel N. Antigenic heterogeneity among influenza A(H3N2) field isolates during an outbreak in 1982/83, estimated by methods of numerical taxonomy. J Hyg (Lond) 1985 Feb;94(1):97–109. doi: 10.1017/s0022172400061179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carroll S. M., Higa H. H., Paulson J. C. Different cell-surface receptor determinants of antigenically similar influenza virus hemagglutinins. J Biol Chem. 1981 Aug 25;256(16):8357–8363. [PubMed] [Google Scholar]
  3. Deom C. M., Caton A. J., Schulze I. T. Host cell-mediated selection of a mutant influenza A virus that has lost a complex oligosaccharide from the tip of the hemagglutinin. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3771–3775. doi: 10.1073/pnas.83.11.3771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lathey J. L., Van Voris L. P., Belshe R. B. Superiority of tissue-culture-grown antigens over egg-grown antigens for serologic diagnosis of influenza B virus infections. J Med Virol. 1986 Jun;19(2):155–159. doi: 10.1002/jmv.1890190208. [DOI] [PubMed] [Google Scholar]
  5. Patterson S., Oxford J. S. Analysis of antigenic determinants on internal and external proteins of influenza virus and identification of antigenic subpopulations of virions in recent field isolates using monoclonal antibodies and immunogold labelling. Arch Virol. 1986;88(3-4):189–202. doi: 10.1007/BF01310874. [DOI] [PubMed] [Google Scholar]
  6. Pyhälä R., Aho K. Serum HI antibody and protection against influenza: a follow-up survey at community level of three epidemics caused by different H3N2-variants. Int J Epidemiol. 1975 Jun;4(2):127–129. doi: 10.1093/ije/4.2.127. [DOI] [PubMed] [Google Scholar]
  7. Pyhälä R., Pyhälä L. Antigenic analysis of intraepidemic variants of influenza A (H3N2) viruses by hyperimmune rat antisera. J Virol Methods. 1987 Mar;15(4):259–265. doi: 10.1016/0166-0934(87)90147-9. [DOI] [PubMed] [Google Scholar]
  8. Pyhälä R., Pyhälä L., Visakorpi R. Intraepidemic heterogeneity of influenza A (H3N2) viruses in 1985: antigenic analysis and sensitivity to non-specific inhibitors. Med Biol. 1986;64(5):277–284. [PubMed] [Google Scholar]
  9. Robertson J. S., Naeve C. W., Webster R. G., Bootman J. S., Newman R., Schild G. C. Alterations in the hemagglutinin associated with adaptation of influenza B virus to growth in eggs. Virology. 1985 May;143(1):166–174. doi: 10.1016/0042-6822(85)90105-9. [DOI] [PubMed] [Google Scholar]
  10. Rogers G. N., Daniels R. S., Skehel J. J., Wiley D. C., Wang X. F., Higa H. H., Paulson J. C. Host-mediated selection of influenza virus receptor variants. Sialic acid-alpha 2,6Gal-specific clones of A/duck/Ukraine/1/63 revert to sialic acid-alpha 2,3Gal-specific wild type in ovo. J Biol Chem. 1985 Jun 25;260(12):7362–7367. [PubMed] [Google Scholar]
  11. Rogers G. N., Paulson J. C. Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin. Virology. 1983 Jun;127(2):361–373. doi: 10.1016/0042-6822(83)90150-2. [DOI] [PubMed] [Google Scholar]
  12. Rogers G. N., Pritchett T. J., Lane J. L., Paulson J. C. Differential sensitivity of human, avian, and equine influenza A viruses to a glycoprotein inhibitor of infection: selection of receptor specific variants. Virology. 1983 Dec;131(2):394–408. doi: 10.1016/0042-6822(83)90507-x. [DOI] [PubMed] [Google Scholar]
  13. Rott R., Orlich M., Klenk H. D., Wang M. L., Skehel J. J., Wiley D. C. Studies on the adaptation of influenza viruses to MDCK cells. EMBO J. 1984 Dec 20;3(13):3329–3332. doi: 10.1002/j.1460-2075.1984.tb02299.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schild G. C., Oxford J. S., de Jong J. C., Webster R. G. Evidence for host-cell selection of influenza virus antigenic variants. Nature. 1983 Jun 23;303(5919):706–709. doi: 10.1038/303706a0. [DOI] [PubMed] [Google Scholar]
  15. Styk B., Kostolanský F., Russ G., Tůmová B. Characterization of influenza A-1983 epidemic strains by polyclonal and monoclonal antibodies and detection of two co-circulating antigenic variants. Acta Virol. 1986 May;30(3):220–227. [PubMed] [Google Scholar]
  16. Tobita K., Kilbourne E. D. Genetic recombination for antigenic markers of antigenically different strains of influenza B virus. J Virol. 1974 Feb;13(2):347–352. doi: 10.1128/jvi.13.2.347-352.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Turner R., Lathey J. L., Van Voris L. P., Belshe R. B. Serological diagnosis of influenza B virus infection: comparison of an enzyme-linked immunosorbent assay and the hemagglutination inhibition test. J Clin Microbiol. 1982 May;15(5):824–829. doi: 10.1128/jcm.15.5.824-829.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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