Abstract
The usefulness of sodium dodecyl sulphate-polyacrylamide gel electrophoresis patterns of cell envelope proteins for classifying strains of Acinetobacter calcoaceticus was studied using 129 isolates from 16 in-patients in a teaching hospital. In 11 patients, all of the isolates from each patient exhibited the same pattern irrespective of the body site or time of isolation. The patterns of the isolates from four other patients were indistinguishable, with the exception of one isolate per patient. In the isolates from one patient five patterns were observed. In several cases isolates from different patients exhibited the same pattern. The relative frequency of some of these patterns was low. Epidemiological data were compatible with the assumption that the concurrent presence of bacteria of these patterns in the patients was the result of cross-infection. For one pattern, which was seen in seven patients, cross-infection could not be substantiated. On the basis of analysis of electrophoretic patterns in combination with epidemiological data on a number of strains it is concluded that cell-envelope protein profiles appear to be a useful aid in studying the dissemination of Acinetobacter in the hospital environment.
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- Alexander M., Ismail F., Jackman P. J., Noble W. C. Fingerprinting Acinetobacter strains from clinical sources by numerical analysis of electrophoretic protein patterns. J Med Microbiol. 1984 Aug;18(1):55–64. doi: 10.1099/00222615-18-1-55. [DOI] [PubMed] [Google Scholar]
- Allen K. D., Green H. T. Hospital outbreak of multi-resistant Acinetobacter anitratus: an airborne mode of spread? J Hosp Infect. 1987 Mar;9(2):110–119. doi: 10.1016/0195-6701(87)90048-x. [DOI] [PubMed] [Google Scholar]
- Andrews H. J. Acinetobacter bacteriocin typing. J Hosp Infect. 1986 Mar;7(2):169–175. doi: 10.1016/0195-6701(86)90060-5. [DOI] [PubMed] [Google Scholar]
- Blaser M. J., Hopkins J. A., Berka R. M., Vasil M. L., Wang W. L. Identification and characterization of Campylobacter jejuni outer membrane proteins. Infect Immun. 1983 Oct;42(1):276–284. doi: 10.1128/iai.42.1.276-284.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Das B. C., Ayliffe G. Serotyping of Acinetobacter calcoaceticus. J Clin Pathol. 1984 Dec;37(12):1388–1391. doi: 10.1136/jcp.37.12.1388. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dijkshoorn L., Michel M. F., Degener J. E. Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals. J Med Microbiol. 1987 Jun;23(4):313–319. doi: 10.1099/00222615-23-4-313. [DOI] [PubMed] [Google Scholar]
- Henriksen S. D. Moraxella, Acinetobacter, and the Mimeae. Bacteriol Rev. 1973 Dec;37(4):522–561. doi: 10.1128/br.37.4.522-561.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holton J. A report of a further hospital outbreak caused by a multi-resistant Acinetobacter anitratus. J Hosp Infect. 1982 Sep;3(3):305–309. doi: 10.1016/0195-6701(82)90051-2. [DOI] [PubMed] [Google Scholar]
- Joly-Guillou M. L., Bergogne-Berezin E., Laban P., Gayral J. P. Etude bioenzymatique et lysotypique de deux cents souches d'Acinetobacter calcoaceticus. Pathol Biol (Paris) 1984 Mar;32(3):177–181. [PubMed] [Google Scholar]
- Loeb M. R., Smith D. H. Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications. Infect Immun. 1980 Dec;30(3):709–717. doi: 10.1128/iai.30.3.709-717.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
- Sparling P. F., Cannon J. G., So M. Phase and antigenic variation of pili and outer membrane protein II of Neisseria gonorrhoeae. J Infect Dis. 1986 Feb;153(2):196–201. doi: 10.1093/infdis/153.2.196. [DOI] [PubMed] [Google Scholar]
- Zak K., Diaz J. L., Jackson D., Heckels J. E. Antigenic variation during infection with Neisseria gonorrhoeae: detection of antibodies to surface proteins in sera of patients with gonorrhea. J Infect Dis. 1984 Feb;149(2):166–174. doi: 10.1093/infdis/149.2.166. [DOI] [PubMed] [Google Scholar]


