Skip to main content
NCBI home page
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1987 Dec;99(3):733–744. doi: 10.1017/s0950268800066590

Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. III. Evaluation of antibodies after infection and vaccination.

C Hamblin 1, R P Kitching 1, A I Donaldson 1, J R Crowther 1, I T Barnett 1
PMCID: PMC2249258  PMID: 3428376

Abstract

Investigations using a liquid-phase blocking sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies against foot-and-mouth disease virus (FMDV) in sera from sheep and from cattle are reported, and results compared with those obtained by virus neutralization (VN) tests. Serum antibody titres in sheep after primary vaccination and in cattle challenged with a natural aerosol after vaccination were similar by ELISA and VN. However, the antibody levels detected in sera of cattle during early infection and of vaccinated cattle after intradermolingual challenge were clearly greater by ELISA than by VN. The ELISA titres in cattle sera following synthetic peptide vaccination indicated some relationship to protection and were clearly different from those recorded by VN. On the other hand, the antibody levels following conventional vaccination showed that ELISA and VN titres in cattle sera were related to protection. Although there was a good agreement between the ELISA antibody titre and protection for the four vaccines used, by VN the titre which afforded protection varied depending on the vaccine used. The ELISA was considered therefore to be more reliable than the VN and may prove useful for evaluating the immunological response of animals following infection and following vaccination.

Full text

PDF
733

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer K., Lorenz R. J., Wittmann G. Untersuchungen zur Abhängigkeit zwischen immunität und Höhe der Neutralisationstiter bei Schweinen nach Impfung mit Maul- und Klauenseuche-(MKS-)Vakzinen. Arch Virol. 1975;49(4):349–357. doi: 10.1007/BF01318244. [DOI] [PubMed] [Google Scholar]
  2. Black L., Francis M. J., Rweyemamu M. M., Umebara O., Boge A. The relationship between serum antibody titres and protection from foot and mouth disease in pigs after oil emulsion vaccination. J Biol Stand. 1984 Oct;12(4):379–389. doi: 10.1016/s0092-1157(84)80062-1. [DOI] [PubMed] [Google Scholar]
  3. DiMarchi R., Brooke G., Gale C., Cracknell V., Doel T., Mowat N. Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science. 1986 May 2;232(4750):639–641. doi: 10.1126/science.3008333. [DOI] [PubMed] [Google Scholar]
  4. Golding S. M., Hedger R. S., Talbot P. Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular disease. Res Vet Sci. 1976 Mar;20(2):142–147. [PubMed] [Google Scholar]
  5. Hamblin C., Barnett I. T., Crowther J. R. A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. II. Application. J Immunol Methods. 1986 Oct 23;93(1):123–129. doi: 10.1016/0022-1759(86)90442-4. [DOI] [PubMed] [Google Scholar]
  6. Hamblin C., Barnett I. T., Hedger R. S. A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method of ELISA. J Immunol Methods. 1986 Oct 23;93(1):115–121. doi: 10.1016/0022-1759(86)90441-2. [DOI] [PubMed] [Google Scholar]
  7. MACKOWIAK C., LANG R., FONTAINE J., CAMAND R., PETERMANN H. G. [Relation between the titer of neutralizing antibodies and the protection of animals following anti-aphthous vaccination]. Ann Inst Pasteur (Paris) 1962 Aug;103:252–261. [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES