Skip to main content
PMC home page
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1989 Feb;102(1):11–19. doi: 10.1017/s0950268800029630

Subtyping of Haemophilus influenzae type b strains from Europe and North America by SDS-PAGE of whole-cell polypeptides: the geographical distribution of subtypes.

T H Pennington 1, E M Freebairn 1
PMCID: PMC2249305  PMID: 2783912

Abstract

One hundred and nine strains of Haemophilus influenzae type b were subtyped by sodium dodecyl sulphate polyacrylamide gel electrophoresis of whole-cell polypeptides. Twenty-one strains from England, 44 from Scotland, 8 from Sweden, 6 from the Netherlands and 30 from the USA were examined. Some of these strains had been subtyped by outer membrane protein analysis; most of the strains had been isolated from cases of invasive disease. Comparison of polypeptide profiles using the Dice coefficient of similarity showed that the majority of European strains were closely related and formed a single large group. Four smaller groups were identified; three of these included American and European strains, indicating a world-wide distribution of subtypes. However, the common European and American subtypes fell into different groups, indicating the existence of marked geographical variations in subtype frequency.

Full text

PDF
11

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barenkamp S. J., Granoff D. M., Pittman M. Outer membrane protein subtypes and biotypes of Haemophilus influenzae type b: relation between strains isolated in 1934-1954 and 1977-1980. J Infect Dis. 1983 Dec;148(6):1127–1127. doi: 10.1093/infdis/148.6.1127. [DOI] [PubMed] [Google Scholar]
  2. Barenkamp S. J., Munson R. S., Jr, Granoff D. M. Comparison of outer-membrane protein subtypes and biotypes of isolates of Haemophilus influenzae type b. J Infect Dis. 1981 Nov;144(5):480–480. doi: 10.1093/infdis/144.5.480. [DOI] [PubMed] [Google Scholar]
  3. Barenkamp S. J., Munson R. S., Jr, Granoff D. M. Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles. J Infect Dis. 1981 May;143(5):668–676. doi: 10.1093/infdis/143.5.668. [DOI] [PubMed] [Google Scholar]
  4. Hampton C. M., Barenkamp S. J., Granoff D. M. Comparison of outer membrane protein subtypes of Haemophilus influenzae type b isolates from healthy children in the general population and from diseased patients. J Clin Microbiol. 1983 Sep;18(3):596–600. doi: 10.1128/jcm.18.3.596-600.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  6. Krikler S. J., Pennington T. H., Petrie D. Typing of strains of Staphylococcus aureus by Western Blot analysis of culture supernates. J Med Microbiol. 1986 Mar;21(2):169–171. doi: 10.1099/00222615-21-2-169. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Musser J. M., Barenkamp S. J., Granoff D. M., Selander R. K. Genetic relationships of serologically nontypable and serotype b strains of Haemophilus influenzae. Infect Immun. 1986 Apr;52(1):183–191. doi: 10.1128/iai.52.1.183-191.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Musser J. M., Granoff D. M., Pattison P. E., Selander R. K. A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5078–5082. doi: 10.1073/pnas.82.15.5078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Paterson A. J., Macsween K. F., Pennington T. H. Haemophilus influenzae subtyping by SDS-PAGE of whole-cell polypeptides. Epidemiol Infect. 1987 Aug;99(1):179–189. doi: 10.1017/s0950268800067017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Porras O., Caugant D. A., Lagergård T., Svanborg-Edén C. Application of multilocus enzyme gel electrophoresis to Haemophilus influenzae. Infect Immun. 1986 Jul;53(1):71–78. doi: 10.1128/iai.53.1.71-78.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. van Alphen L., Geelen L., Jónsdóttir K., Takala A. K., Käyhty H., Zanen H. C. Distinct geographic distribution of subtypes of Haemophilus influenzae type b in Western Europe. J Infect Dis. 1987 Jul;156(1):216–218. doi: 10.1093/infdis/156.1.216. [DOI] [PubMed] [Google Scholar]
  13. van Alphen L., Riemens T., Poolman J., Hopman C., Zanen H. C. Homogeneity of cell envelope protein subtypes, lipopolysaccharide serotypes, and biotypes among Haemophilus influenzae type b from patients with meningitis in The Netherlands. J Infect Dis. 1983 Jul;148(1):75–81. doi: 10.1093/infdis/148.1.75. [DOI] [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES