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. 1989 Feb;102(1):37–46. doi: 10.1017/s0950268800029666

Genetic relationships among Escherichia coli isolates causing urinary tract infections in humans and animals.

T S Whittam 1, M L Wolfe 1, R A Wilson 1
PMCID: PMC2249307  PMID: 2645153

Abstract

Genetic variation in isolates of Escherichia coli obtained mostly from urinary tract infections in humans and domesticated animals (dogs and cats) was assessed for 16 enzymes using multilocus enzyme electrophoresis to characterize chromosomal genotypes. A total of 148 isolates comprised 63 distinct electrophoretic types (ETs) and about half of the isolates belonged to one of 9 common ETs. A bootstrap analysis of genetic distance between ETs revealed three significant groups of strains. Variation in allele frequencies among groups accounted for 40% of the total genetic diversity. The majority of the common ETs fell into a major cluster of closely related strains. The recovery of multiple isolates of the same electrophoretic types and serotypes from unassociated hosts suggests that these bacteria represent uropathogenic clones that are widely disseminated in humans and animals.

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Selected References

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  1. Achtman M., Heuzenroeder M., Kusecek B., Ochman H., Caugant D., Selander R. K., Väisanen-Rhen V., Korhonen T. K., Stuart S., Orskov F. Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. Infect Immun. 1986 Jan;51(1):268–276. doi: 10.1128/iai.51.1.268-276.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R. P. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun. 1983 Jan;39(1):315–335. doi: 10.1128/iai.39.1.315-335.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Achtman M., Pluschke G. Clonal analysis of descent and virulence among selected Escherichia coli. Annu Rev Microbiol. 1986;40:185–210. doi: 10.1146/annurev.mi.40.100186.001153. [DOI] [PubMed] [Google Scholar]
  4. Belsheim J., Gnarpe H., Kaijser B. Chemoattractive factors from Escherichia coli: individual strain variation. Infect Immun. 1981 Jan;31(1):7–10. doi: 10.1128/iai.31.1.7-10.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caugant D. A., Levin B. R., Lidin-Janson G., Whittam T. S., Svanborg Edén C., Selander R. K. Genetic diversity and relationships among strains of Escherichia coli in the intestine and those causing urinary tract infections. Prog Allergy. 1983;33:203–227. doi: 10.1159/000318331. [DOI] [PubMed] [Google Scholar]
  6. Cooke E. M., Ewins S. P. Properties of strains of Escherichia coli isolated from a variety of sources. J Med Microbiol. 1975 Feb;8(1):107–111. doi: 10.1099/00222615-8-1-107. [DOI] [PubMed] [Google Scholar]
  7. Garcia E., Bergmans H. E., Van den Bosch J. F., Orskov I., Van der Zeijst B. A., Gaastra W. Isolation and characterisation of dog uropathogenic Escherichia coli strains and their fimbriae. Antonie Van Leeuwenhoek. 1988;54(2):149–163. doi: 10.1007/BF00419202. [DOI] [PubMed] [Google Scholar]
  8. Grüneberg R. N., Bettelheim K. A. Geographical variation in serological types of urinary Escherichia coli. J Med Microbiol. 1969 Aug;2(3):219–224. doi: 10.1099/00222615-2-3-219. [DOI] [PubMed] [Google Scholar]
  9. Hughes C., Hacker J., Roberts A., Goebel W. Hemolysin production as a virulence marker in symptomatic and asymptomatic urinary tract infections caused by Escherichia coli. Infect Immun. 1983 Feb;39(2):546–551. doi: 10.1128/iai.39.2.546-551.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaijser B., Hanson L. A., Jodal U., Lidin-Janson G., Robbins J. B. Frequency of E. coli K antigens in urinary-tract infections in children. Lancet. 1977 Mar 26;1(8013):663–666. doi: 10.1016/s0140-6736(77)92111-0. [DOI] [PubMed] [Google Scholar]
  11. Källenius G., Möllby R., Svenson S. B., Helin I., Hultberg H., Cedergren B., Winberg J. Occurrence of P-fimbriated Escherichia coli in urinary tract infections. Lancet. 1981 Dec 19;2(8260-61):1369–1372. doi: 10.1016/s0140-6736(81)92797-5. [DOI] [PubMed] [Google Scholar]
  12. Mabeck C. E., Orskov F., Orskov I. Escherichia coli serotypes and renal involvement in urinary-tract infection. Lancet. 1971 Jun 26;1(7713):1312–1314. doi: 10.1016/s0140-6736(71)91884-8. [DOI] [PubMed] [Google Scholar]
  13. Ochman H., Selander R. K. Evidence for clonal population structure in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jan;81(1):198–201. doi: 10.1073/pnas.81.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Orskov I., Orskov F., Birch-Andersen A., Kanamori M., Svanborg-Edén C. O, K, H and fimbrial antigens in Escherichia coli serotypes associated with pyelonephritis and cystitis. Scand J Infect Dis Suppl. 1982;33:18–25. [PubMed] [Google Scholar]
  15. Orskov I., Orskov F., Jann B., Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977 Sep;41(3):667–710. doi: 10.1128/br.41.3.667-710.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Roberts A. P., Linton J. D., Waterman A. M., Gower P. E., Koutsaimanis K. G. Urinary and faecal Escherichia coli O-sero-groups in symptomatic urinary-tract infection and asymptomatic bacteriuria. J Med Microbiol. 1975 May;8(2):311–318. doi: 10.1099/00222615-8-2-311. [DOI] [PubMed] [Google Scholar]
  17. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Selander R. K., Korhonen T. K., Väisänen-Rhen V., Williams P. H., Pattison P. E., Caugant D. A. Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infect Immun. 1986 Apr;52(1):213–222. doi: 10.1128/iai.52.1.213-222.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stenderup J., Orskov F. The clonal nature of enteropathogenic Escherichia coli strains. J Infect Dis. 1983 Dec;148(6):1019–1024. doi: 10.1093/infdis/148.6.1019. [DOI] [PubMed] [Google Scholar]
  20. Väisänen-Rhen V., Elo J., Väisänen E., Siitonen A., Orskov I., Orskov F., Svenson S. B., Mäkelä P. H., Korhonen T. K. P-fimbriated clones among uropathogenic Escherichia coli strains. Infect Immun. 1984 Jan;43(1):149–155. doi: 10.1128/iai.43.1.149-155.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Westerlund B., Pere A., Korhonen T. K., Jarvinen A. K., Siitonen A., Williams P. H. Characterisation of Escherichia coli strains associated with canine urinary tract infections. Res Vet Sci. 1987 May;42(3):404–406. [PubMed] [Google Scholar]
  22. Whittam T. S., Ochman H., Selander R. K. Multilocus genetic structure in natural populations of Escherichia coli. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1751–1755. doi: 10.1073/pnas.80.6.1751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Whittam T. S., Wachsmuth I. K., Wilson R. A. Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis. 1988 Jun;157(6):1124–1133. doi: 10.1093/infdis/157.6.1124. [DOI] [PubMed] [Google Scholar]
  24. Whittam T. S., Wilson R. A. Genetic relationships among pathogenic Escherichia coli of serogroup O157. Infect Immun. 1988 Sep;56(9):2467–2473. doi: 10.1128/iai.56.9.2467-2473.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Whittam T. S., Wilson R. A. Genetic relationships among pathogenic strains of avian Escherichia coli. Infect Immun. 1988 Sep;56(9):2458–2466. doi: 10.1128/iai.56.9.2458-2466.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilson R. A., Keefe T. J., Davis M. A., Browning M. T., Ondrusek K. Strains of Escherichia coli associated with urogenital disease in dogs and cats. Am J Vet Res. 1988 Jun;49(6):743–746. [PubMed] [Google Scholar]

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