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. 1988 Oct;101(2):445–458. doi: 10.1017/s0950268800054406

Restriction enzyme analysis of faecal adenoviruses in Newcastle upon Tyne.

M M Willcocks 1, M J Carter 1, F R Laidler 1, C R Madeley 1
PMCID: PMC2249390  PMID: 2460364

Abstract

Adenovirus DNA was isolated directly from virus-containing stools and digested with restriction endonucleases. The resulting fragments were separated by polyacrylamide gel electrophoresis (PAGE) and visualized by silver staining. This enabled us to assign most of the viruses detected to subgenus, serotype and, sometimes, unique strains. Although less sensitive than electron microscopy, the method allowed more information about the infecting virus to be obtained and no cultivation was necessary. Comparison with culture also allowed dual infections to be recognized. A 2-year survey of faecal adenoviruses in Newcastle upon Tyne showed that type 41 (strain 41a) was the predominant type and strain 41p was not recorded. Heterogeneity in strain 41a was also noted as found elsewhere. Adenovirus type 40 was common prior to 1985 but was absent during the last 2 years.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian T., Wadell G., Hierholzer J. C., Wigand R. DNA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol. 1986;91(3-4):277–290. doi: 10.1007/BF01314287. [DOI] [PubMed] [Google Scholar]
  2. Brandt C. D., Kim H. W., Yolken R. H., Kapikian A. Z., Arrobio J. O., Rodriguez W. J., Wyatt R. G., Chanock R. M., Parrott R. H. Comparative epidemiology of two rotavirus serotypes and other viral agents associated with pediatric gastroenteritis. Am J Epidemiol. 1979 Sep;110(3):243–254. doi: 10.1093/oxfordjournals.aje.a112809. [DOI] [PubMed] [Google Scholar]
  3. Brown M., Petric M., Middleton P. J. Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimens. J Clin Microbiol. 1984 Sep;20(3):334–338. doi: 10.1128/jcm.20.3.334-338.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown M., Petric M., Middleton P. J. Silver staining of DNA restriction fragments for the rapid identification of adenovirus isolates: application during nosocomial outbreaks. J Virol Methods. 1984 Oct;9(2):87–98. doi: 10.1016/0166-0934(84)90001-6. [DOI] [PubMed] [Google Scholar]
  5. Buitenwerf J., Louwerens J. J., De Jong J. C. A simple and rapid method for typing adenoviruses 40 and 41 without cultivation. J Virol Methods. 1985 Jan;10(1):39–44. doi: 10.1016/0166-0934(85)90086-2. [DOI] [PubMed] [Google Scholar]
  6. Flewett T. H. Diagnosis of enteritis virus. Proc R Soc Med. 1976 Sep;69(9):693–696. doi: 10.1177/003591577606900920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green M., Mackey J. K., Wold W. S., Rigden P. Thirty-one human adenovirus serotypes (Ad1-Ad31) form five groups (A-E) based upon DNA genome homologies. Virology. 1979 Mar;93(2):481–492. doi: 10.1016/0042-6822(79)90251-4. [DOI] [PubMed] [Google Scholar]
  8. Hammond G., Hannan C., Yeh T., Fischer K., Mauthe G., Straus S. E. DNA hybridization for diagnosis of enteric adenovirus infection from directly spotted human fecal specimens. J Clin Microbiol. 1987 Oct;25(10):1881–1885. doi: 10.1128/jcm.25.10.1881-1885.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kidd A. H., Banatvala J. E., de Jong J. C. Antibodies to fastidious faecal adenoviruses (species 40 and 41) in sera from children. J Med Virol. 1983;11(4):333–341. doi: 10.1002/jmv.1890110409. [DOI] [PubMed] [Google Scholar]
  11. Kidd A. H. Genome variants of adenovirus 41 (subgroup G) from children with diarrhoea in South Africa. J Med Virol. 1984;14(1):49–59. doi: 10.1002/jmv.1890140108. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Moosai R. B., Carter M. J., Madeley C. R. Rapid detection of enteric adenovirus and rotavirus: a simple method using polyacrylamide gel electrophoresis. J Clin Pathol. 1984 Dec;37(12):1404–1408. doi: 10.1136/jcp.37.12.1404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Okayama H., Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. doi: 10.1128/mcb.2.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Richmond S. J., Wood D. J., Bailey A. S. Recent respiratory and enteric adenovirus infection in children in the Manchester area. J R Soc Med. 1988 Jan;81(1):15–18. doi: 10.1177/014107688808100108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rose J. A., Hoggan M. D., Koczot F., Shatkin A. J. Genetic relatedness studies with adenovirus-associated viruses. J Virol. 1968 Oct;2(10):999–1005. doi: 10.1128/jvi.2.10.999-1005.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmitz H., Wigand R., Heinrich W. Worldwide epidemiology of human adenovirus infections. Am J Epidemiol. 1983 Apr;117(4):455–466. doi: 10.1093/oxfordjournals.aje.a113563. [DOI] [PubMed] [Google Scholar]
  18. Takiff H. E., Reinhold W., Garon C. F., Straus S. E. Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41). J Virol. 1984 Jul;51(1):131–136. doi: 10.1128/jvi.51.1.131-136.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Takiff H. E., Straus S. E., Garon C. F. Propagation and in vitro studies of previously non-cultivable enteral adenoviruses in 293 cells. Lancet. 1981 Oct 17;2(8251):832–834. doi: 10.1016/s0140-6736(81)91104-1. [DOI] [PubMed] [Google Scholar]
  20. Wadell G., Cooney M. K., da Costa Linhares A., de Silva L., Kennett M. L., Kono R., Gui-Fang R., Lindman K., Nascimento J. P., Schoub B. D. Molecular epidemiology of adenoviruses: global distribution of adenovirus 7 genome types. J Clin Microbiol. 1985 Mar;21(3):403–408. doi: 10.1128/jcm.21.3.403-408.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wadell G. Molecular epidemiology of human adenoviruses. Curr Top Microbiol Immunol. 1984;110:191–220. doi: 10.1007/978-3-642-46494-2_7. [DOI] [PubMed] [Google Scholar]
  22. de Jong J. C., Wigand R., Kidd A. H., Wadell G., Kapsenberg J. G., Muzerie C. J., Wermenbol A. G., Firtzlaff R. G. Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. J Med Virol. 1983;11(3):215–231. doi: 10.1002/jmv.1890110305. [DOI] [PubMed] [Google Scholar]

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