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. 1989 Apr;102(2):281–285. doi: 10.1017/s0950268800029952

Recovery of Campylobacter from human faeces stored at 4 degrees C.

C Ladrón de Guevara 1, M T Pérez-Pomata 1, A Agulla 1, F J Merino 1, P A Villasante 1, A C Velasco 1
PMCID: PMC2249446  PMID: 2703021

Abstract

Six hundred and thirteen fresh diarrhoeal faeces were inoculated on Skirrow blood agar (SK), on Preston blood free agar (PBF), and in Campy-thioglycolate broth (CT). After 24 h of storage at 4 degrees C, specimens were again inoculated on SK and PBF, and in Campylobacter enrichment broth (CEB). CT tubes were placed overnight at 4 degrees C. Plates and CEB tubes were incubated at 43 degrees C in microaerophilic conditions. A total of 68 specimens was positive for campylobacter on direct plating. Sixty-four of them were also recovered after subculturing from CT, and only 51 from CEB. Delayed inoculation of plates after storage of samples at 4 degrees C yielded 57 isolates. The storage of faeces at 4 degrees C for 24 h significantly reduces the number of campylobacter isolates. When samples are not plated immediately we recommend inoculating a CT tube maintained at 4 degrees C overnight as a holding medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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