Skip to main content
PMC home page
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1989 Aug;103(1):165–171. doi: 10.1017/s0950268800030466

DNA fingerprinting of Streptococcus uberis: a useful tool for epidemiology of bovine mastitis.

A W Hill 1, J A Leigh 1
PMCID: PMC2249475  PMID: 2776850

Abstract

A simple and reproducible typing system based on restriction fragment size of chromosomal DNA was developed to compare isolates of Streptococcus uberis obtained from the bovine mammary gland. The endonuclease giving the most useful restriction patterns was Hind III. Although seven other endonucleases (Bgl 1, Eco R1, Not 1, Pst 1, Sfi 1, Sma 1, Xba 1) were also tested in the system. An image analyser was used to obtain a densitometric scan and a graphic display of the restriction patterns. Such a system will allow large scale data storage for future computer-aided comparison.

Full text

PDF
165

Images in this article

168Fig. 1
on p.168
169Fig. 2
on p.169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bramley A. J., Dodd F. H. Reviews of the progress of dairy science: mastitis control--progress and prospects. J Dairy Res. 1984 Aug;51(3):481–512. doi: 10.1017/s0022029900023797. [DOI] [PubMed] [Google Scholar]
  2. Burnie J. P., Lee W. A comparison of DNA and immunoblot fingerprinting of the SII biotype of coagulase negative staphylococci. Epidemiol Infect. 1988 Oct;101(2):203–212. doi: 10.1017/s095026880005411x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kakoyiannis C. K., Winter P. J., Marshall R. B. Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis. Appl Environ Microbiol. 1984 Sep;48(3):545–549. doi: 10.1128/aem.48.3.545-549.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kakoyiannis C. K., Winter P. J., Marshall R. B. The relationship between intestinal Campylobacter species isolated from animals and humans as determined by BRENDA. Epidemiol Infect. 1988 Jun;100(3):379–387. doi: 10.1017/s0950268800067133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaper J. B., Bradford H. B., Roberts N. C., Falkow S. Molecular epidemiology of Vibrio cholerae in the U.S. Gulf Coast. J Clin Microbiol. 1982 Jul;16(1):129–134. doi: 10.1128/jcm.16.1.129-134.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Marshall R. B., Wilton B. E., Robinson A. J. Identification of Leptospira serovars by restriction-endonuclease analysis. J Med Microbiol. 1981 Feb;14(1):163–166. doi: 10.1099/00222615-14-1-163. [DOI] [PubMed] [Google Scholar]
  7. Marshall R. B., Winter P. J., Cooper B. S., Robinson A. J. Subspecies differentiation of Moraxella bovis by restriction endonuclease DNA analysis (BRENDA). N Z Vet J. 1985 May;33(5):67–70. doi: 10.1080/00480169.1985.35167. [DOI] [PubMed] [Google Scholar]
  8. Miller S. A., Dykes D. D., Polesky H. F. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988 Feb 11;16(3):1215–1215. doi: 10.1093/nar/16.3.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tagg J. R., Vugler L. G. An inhibitor typing scheme for Streptococcus uberis. J Dairy Res. 1986 Aug;53(3):451–456. doi: 10.1017/s0022029900025061. [DOI] [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES