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. 1989 Aug;103(1):105–112. doi: 10.1017/s0950268800030405

Detection of Legionella pneumophila in environmental water samples using a fluorescein conjugated monoclonal antibody.

T Makin 1, C A Hart 1
PMCID: PMC2249478  PMID: 2673821

Abstract

Sixty-three environmental water samples from various sources were examined for the presence of Legionella pneumophila with a commercially available direct fluorescent monoclonal antibody (GS), an indirect fluorescent antibody test (IFAT) and culture. GS detected L. pneumophila in 94% and 100% of environmental water samples which were culture and IFAT positive for L. pneumophila, respectively. IFAT detected 69% of L. pneumophila culture positive samples. Cultures of L. pneumophila serogroups 1 to 12, 14 and non-L. pneumophila bacteria which may be found in water, and bacteria containing non-specific binding proteins, were stained by GS and IFAT. GS identified all serogroups of L. pneumophila and did not cross react with any non-L. pneumophila bacteria. L. pneumophila in environmental samples was easy to detect against a clear dark background when stained with GS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benson R. F., Thacker W. L., Wilkinson H. W., Fallon R. J., Brenner D. J. Legionella pneumophila serogroup 14 isolated from patients with fatal pneumonia. J Clin Microbiol. 1988 Feb;26(2):382–382. doi: 10.1128/jcm.26.2.382-.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dennis P. J., Taylor J. A., Fitzgeorge R. B., Bartlett C. L., Barrow G. I. Legionella pneumophila in water plumbing systems. Lancet. 1982 Apr 24;1(8278):949–951. doi: 10.1016/s0140-6736(82)91944-4. [DOI] [PubMed] [Google Scholar]
  3. Edelstein P. H., Beer K. B., Sturge J. C., Watson A. J., Goldstein L. C. Clinical utility of a monoclonal direct fluorescent reagent specific for Legionella pneumophila: comparative study with other reagents. J Clin Microbiol. 1985 Sep;22(3):419–421. doi: 10.1128/jcm.22.3.419-421.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelstein P. H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol. 1981 Sep;14(3):298–303. doi: 10.1128/jcm.14.3.298-303.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fallon R. J. Identification of Legionella pneumophila with commercially available immunofluorescence test. J Clin Pathol. 1986 Jun;39(6):693–694. doi: 10.1136/jcp.39.6.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fliermans C. B., Cherry W. B., Orrison L. H., Smith S. J., Tison D. L., Pope D. H. Ecological distribution of Legionella pneumophila. Appl Environ Microbiol. 1981 Jan;41(1):9–16. doi: 10.1128/aem.41.1.9-16.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldberg D. J., Wrench J. G., Collier P. W., Emslie J. A., Fallon R. J., Forbes G. I., McKay T. M., Macpherson A. C., Markwick T. A., Reid D. Lochgoilhead fever: outbreak of non-pneumonic legionellosis due to Legionella micdadei. Lancet. 1989 Feb 11;1(8633):316–318. doi: 10.1016/s0140-6736(89)91319-6. [DOI] [PubMed] [Google Scholar]
  8. Gosting L. H., Cabrian K., Sturge J. C., Goldstein L. C. Identification of a species-specific antigen in Legionella pneumophila by a monoclonal antibody. J Clin Microbiol. 1984 Dec;20(6):1031–1035. doi: 10.1128/jcm.20.6.1031-1035.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harrison T. G., Taylor A. G. Diagnosis of Legionella pneumophila infections by means of formolised yolk sac antigens. J Clin Pathol. 1982 Feb;35(2):211–214. doi: 10.1136/jcp.35.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McKinney R. M., Wilkinson H. W., Sommers H. M., Fikes B. J., Sasseville K. R., Yungbluth M. M., Wolf J. S. Legionella pneumophila serogroup six: isolation from cases of legionellosis, identification by immunofluorescence staining, and immunological response to infection. J Clin Microbiol. 1980 Sep;12(3):395–401. doi: 10.1128/jcm.12.3.395-401.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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