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. 1968 Oct;61(2):636–643. doi: 10.1073/pnas.61.2.636

Selective enzyme purification by affinity chromatography.

P Cuatrecasas, M Wilchek, C B Anfinsen
PMCID: PMC225207  PMID: 4971842

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARSENIS C., MCCORMICK D. B. PURIFICATION OF LIVER FLAVOKINASE BY COLUMN CHROMATOGRAPHY ON FLAVIN-CELLULOSE COMPOUNDS. J Biol Chem. 1964 Sep;239:3093–3097. [PubMed] [Google Scholar]
  2. BAUTZ E. K., HALL B. D. The isolation of T4-specific RNA on a DNA-cellulose column. Proc Natl Acad Sci U S A. 1962 Mar 15;48:400–408. doi: 10.1073/pnas.48.3.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cuatrecasas P., Fuchs S., Anfinsen C. B. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus. J Biol Chem. 1967 Apr 10;242(7):1541–1547. [PubMed] [Google Scholar]
  4. ERHAN S., NORTHRUP L. G., LEACH F. R. A METHOD POTENTIALLY USEFUL FOR ESTABLISHING BASE SEQUENCES IN CODEWORDS. Proc Natl Acad Sci U S A. 1965 Mar;53:646–652. doi: 10.1073/pnas.53.3.646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fuchs S., Cuatrecasas P., Anfinsen C. B. An improved method for the purification of staphylococcal nuclease. J Biol Chem. 1967 Oct 25;242(20):4768–4770. [PubMed] [Google Scholar]
  6. HUMMEL B. C. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem Physiol. 1959 Dec;37:1393–1399. [PubMed] [Google Scholar]
  7. Lerman L. S. A Biochemically Specific Method for Enzyme Isolation. Proc Natl Acad Sci U S A. 1953 Apr;39(4):232–236. doi: 10.1073/pnas.39.4.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MOUDGAL N. R., PORTER R. R. The use of antigen-cellulose suspensions for the isolation of specific antibodies. Biochim Biophys Acta. 1963 Apr 2;71:185–187. doi: 10.1016/0006-3002(63)90998-3. [DOI] [PubMed] [Google Scholar]
  9. McCormick D. B. Specific purification of avidin by column chromatography on biotin-cellulose. Anal Biochem. 1965 Nov;13(2):194–198. doi: 10.1016/0003-2697(65)90189-2. [DOI] [PubMed] [Google Scholar]
  10. Porath J., Axen R., Ernback S. Chemical coupling of proteins to agarose. Nature. 1967 Sep 30;215(5109):1491–1492. doi: 10.1038/2151491a0. [DOI] [PubMed] [Google Scholar]
  11. Sander E. G., McCormick D. B., Wright L. D. Column chromatography of nucleotides over thymidylate-cellulose. J Chromatogr. 1966 Mar;21(3):419–423. doi: 10.1016/s0021-9673(01)91335-4. [DOI] [PubMed] [Google Scholar]
  12. Silman I., Katchalski E. Water-insoluble derivatives of enzymes, antigens, and antibodies. Annu Rev Biochem. 1966;35:873–908. doi: 10.1146/annurev.bi.35.070166.004301. [DOI] [PubMed] [Google Scholar]
  13. Taniuchi H., Anfinsen C. B., Sodja A. Nuclease-T: an active derivative of staphylococcal nuclease composed of two noncovalently bonded peptide fragments. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1235–1242. doi: 10.1073/pnas.58.3.1235. [DOI] [PMC free article] [PubMed] [Google Scholar]

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