Fig. 3.

Transfection of a 10 L HEK293-EBNA cells culture in a Wave Bioreactor. In two experiments (open symbols experiment 1, closed symbols experiment 2) cells were seeded in Wave bags at a density of 1 × 10⁶ cells mL⁻¹. Two hours later the cultures were transfected and after an additional 4 h incubation, the cultures were fed to the final culture volume. IgG concentration (○●) was determined by ELISA and the viable cell concentration (△▲) and viability (□■) were assessed using a Cedex automatic cell counter