Fig. 2.

Hydrogel casting methods. (a) Computer aided design of the wax mold for the non-adhesive hydrogel. Inset: A close up of the 400 micron pegs. (b) Agarose was poured into a PDMS replicate of the wax mold, shown in image (c), and removed once the agarose had solidified. The resulting gel, seeded with cells at low density, is shown in image (d). The numbers indicate the number of cells settled to the bottom of each recess. This image contains four single-cell wells (denoted by “1”), two double-cell wells (“2”), and one quadruple-cell well (“4”). Scale bar is 200 μm