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. Author manuscript; available in PMC: 2008 Mar 4.
Published in final edited form as: Biomaterials. 2005 Sep 6;27(8):1507–1518. doi: 10.1016/j.biomaterials.2005.08.003

Fig. 3.

Fig. 3

Histological analysis of valvular GAGs using Alcian blue staining. Sections shown are taken from the center of fresh porcine aortic valve cusps (A) and after Glut fixation and storage for 7 days (B), 1 year (C), and 5 years (D). Note the almost complete disappearance of Alcian blue-stainable tissue components after prolonged storage in Glut. Also shown are fresh (E) and 7 day Glut-fixed (F) cusps following treatment with the optimized hyaluronidase/chondroitinase enzyme mixture. Collagen and cells stain red, GAGs stain blue. Original magnification, 100 ×.