Table 1.
Reactivity of purified Ab2 (D9D8) with heterologous sera as the source of Ab11
| Immunizing agent | Species | Titre of Ab1 in immune in sera2 |
|---|---|---|
| Recombinant Baculo H protein (soluble) | Cattle | 6·4 |
| H protein displayed on recombinant extracellular baculovirus | Cattle | > 3·2 |
| Recombinant baculo H protein (membrane-bound form) | Rabbit | 3·2 |
| Peste des petits ruminants virus | Goat3 | > 6·4 |
| Rinderpest virus | Cattle4 | > 3·2 |
1
Microtitre plates were coated with 200 ng/well of Ab2 and dilutions of H-specific immune sera from different sources was added.
2
Data shown are titre (× 103) of Ab1 recognized by Ab2 and are calculated from average optical density (OD) of triplicate wells on comparison with OD of respective dilutions of pre-immune sera. Normal rabbit, bovine and goat serum were used as controls and the OD values of controls were subtracted from the experimental values.
3
Hyper-immunized goats.
4
Convalescent cattle.