Figure 7.

BMP4 inhibits the differentiation of human CD34⁺ thymic precursors. Chimeric hu-mo FTOC were grown in the absence (Control) or presence of BMP4 (100 ng/ml).(a)Dot plots show CD4 versus CD8 expression on gated human CD45⁺ cells from 12-day cultures. Histograms represent the percentage of cycling cells (cells in S plus G2 plus M phases of the cell cycle) as well as the proportion of Annexin V-positive cells in gated human CD4⁺ CD8⁺ thymocytes obtained in these cultures. Data are representative of three independent experiments where the number of human cells recovered was 81 × 10³ and 17 × 10³, in control and treated cultures, respectively.(b)The scatter plot shows the human cell recovery from chimeric hu-mo FTOC cultured for 5 days under the same conditions as described above. The number of human cells recovered in each experiment was divided by the control mean number of human cells recovered, calculated from the six data sets obtained in the control cultures, to give the relative cell number of six individual experiments.(c)Dot plots show the expression of CD4 and CD5 on gated human CD45⁺ cells from hu-mo FTOC cultured for 5 days. Data are representative of three independent experiments.