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Forward- and side-scatter profiles and CD14+ gating of the cells. (a) Monocytes were selected on the basis of their forward- and side-scatter profiles. (b, c) The side scatter gain was then appropriately lowered and the threshold for forward- and side-scatter was increased to exclude lymphocytes, dead cells and cellular debris and the cells that stained positive for CD14 (as compared with the CD3-stained control) were gated to analyse cytokine or surface markers expression.
