Fig. 5.

Deletion analysis of OsPR10a promoter: a schematic diagrams of serial deletion constructs of OsPR10a promoter. The numbers to the left of each construct indicate the distance from the start codon ATG. The predicted cis-elements ( W-box, RAV1AAT, and ASF1motif) are indicated by their respective abbreviations. The start codon, ATG, is written in bold. b Luciferase activity in deletion constructs of the OsPR10a promoter. Each deletion construct OsPR10a:LUC was bombarded into rice leaves, which were incubated in MS liquid medium or MS medium containing 1 mM SA at 28°C for 24 h. Protein extracts were made by dissociation in passive lysis buffer as described in “Materials and methods”. Bars indicate the standard error of three replicates. The values are the ratio of the value obtained from each deletion constructs of OsPR10a promoter treated with SA or buffer divided by the value obtained from 1.0 kb OsPR10a promoter construct treated with buffer