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. 1994 Aug;113(1):153–159. doi: 10.1017/s0950268800051578

Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981-93.

J Noel 1, D Cubitt 1
PMCID: PMC2271231  PMID: 8062872

Abstract

An enzyme immunoassay (EIA) for astrovirus type 1 together with immune electronmicroscopy (IEM) was used to type a collection of 162 astroviruses obtained from 1981-93 from children with diarrhoea. The EIA was found to be specific for astrovirus type 1. Astrovirus types 2-4 were typed by IEM. Astrovirus type 1 was the prevalent serotype 107/125 (86%), followed by type 3 (8%), type 4 (6%) and type 2 (1%). Six samples containing astrovirus could not be typed or detected by EIA because they were coated with coproantibodies; 11 others were not identified. Virus particles could no longer be detected in 15/162 (9%) samples following storage for > or = 2 years. Selected samples containing astrovirus types 1-4 were passaged in CaCO2 cells and their identity confirmed by one or both assays. One sample was shown to have remained viable for 10 years when stored as an aqueous suspension at -20 degrees C. Two patients with severe combined immune deficiency disease (SCID) were shown to be excreting astrovirus type 1 for 32 and 102 days respectively. One child was simultaneously shedding rotavirus and the other child was excreting adenovirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleton H., Higgins P. G. Letter: Viruses and gastroenteritis in infants. Lancet. 1975 Jun 7;1(7919):1297–1297. doi: 10.1016/s0140-6736(75)92581-7. [DOI] [PubMed] [Google Scholar]
  2. Cruz J. R., Bartlett A. V., Herrmann J. E., Cáceres P., Blacklow N. R., Cano F. Astrovirus-associated diarrhea among Guatemalan ambulatory rural children. J Clin Microbiol. 1992 May;30(5):1140–1144. doi: 10.1128/jcm.30.5.1140-1144.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cubitt W. D., McSwiggan D. A., Moore W. Winter vomiting disease caused by calicivirus. J Clin Pathol. 1979 Aug;32(8):786–793. doi: 10.1136/jcp.32.8.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Herrmann J. E., Hudson R. W., Perron-Henry D. M., Kurtz J. B., Blacklow N. R. Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. J Infect Dis. 1988 Jul;158(1):182–185. doi: 10.1093/infdis/158.1.182. [DOI] [PubMed] [Google Scholar]
  5. Herrmann J. E., Nowak N. A., Perron-Henry D. M., Hudson R. W., Cubitt W. D., Blacklow N. R. Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies. J Infect Dis. 1990 Feb;161(2):226–229. doi: 10.1093/infdis/161.2.226. [DOI] [PubMed] [Google Scholar]
  6. Herrmann J. E., Taylor D. N., Echeverria P., Blacklow N. R. Astroviruses as a cause of gastroenteritis in children. N Engl J Med. 1991 Jun 20;324(25):1757–1760. doi: 10.1056/NEJM199106203242501. [DOI] [PubMed] [Google Scholar]
  7. Kurtz J. B., Lee T. W., Craig J. W., Reed S. E. Astrovirus infection in volunteers. J Med Virol. 1979;3(3):221–230. doi: 10.1002/jmv.1890030308. [DOI] [PubMed] [Google Scholar]
  8. Kurtz J. B., Lee T. W. Human astrovirus serotypes. Lancet. 1984 Dec 15;2(8416):1405–1405. doi: 10.1016/s0140-6736(84)92101-9. [DOI] [PubMed] [Google Scholar]
  9. Lee T. W., Kurtz J. B. Astroviruses detected by immunofluorescence. Lancet. 1977 Aug 20;2(8034):406–406. doi: 10.1016/s0140-6736(77)90338-5. [DOI] [PubMed] [Google Scholar]
  10. Lee T. W., Kurtz J. B. Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes. Epidemiol Infect. 1994 Feb;112(1):187–193. doi: 10.1017/s0950268800057551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lee T. W., Kurtz J. B. Serial propagation of astrovirus in tissue culture with the aid of trypsin. J Gen Virol. 1981 Dec;57(Pt 2):421–424. doi: 10.1099/0022-1317-57-2-421. [DOI] [PubMed] [Google Scholar]
  12. Madeley C. R., Cosgrove B. P. Letter: 28 nm particles in faeces in infantile gastroenteritis. Lancet. 1975 Sep 6;2(7932):451–452. doi: 10.1016/s0140-6736(75)90858-2. [DOI] [PubMed] [Google Scholar]
  13. Madeley C. R., Cosgrove B. P. Letter: Viruses in infantile gastroenteritis. Lancet. 1975 Jul 19;2(7925):124–124. doi: 10.1016/s0140-6736(75)90020-3. [DOI] [PubMed] [Google Scholar]
  14. Major M. E., Eglin R. P., Easton A. J. 3' terminal nucleotide sequence of human astrovirus type 1 and routine detection of astrovirus nucleic acid and antigens. J Virol Methods. 1992 Sep;39(1-2):217–225. doi: 10.1016/0166-0934(92)90140-9. [DOI] [PubMed] [Google Scholar]
  15. Willcocks M. M., Carter M. J., Laidler F. R., Madeley C. R. Growth and characterisation of human faecal astrovirus in a continuous cell line. Arch Virol. 1990;113(1-2):73–81. doi: 10.1007/BF01318354. [DOI] [PubMed] [Google Scholar]
  16. Willcocks M. M., Carter M. J., Silcock J. G., Madeley C. R. A dot-blot hybridization procedure for the detection of astrovirus in stool samples. Epidemiol Infect. 1991 Oct;107(2):405–410. doi: 10.1017/s0950268800049049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wilson S. A., Cubitt W. D. The development and evaluation of radioimmune assays for the detection of immune globulins M and G against astrovirus. J Virol Methods. 1988 Feb;19(2):151–159. doi: 10.1016/0166-0934(88)90158-9. [DOI] [PubMed] [Google Scholar]
  18. Wood D. J., David T. J., Chrystie I. L., Totterdell B. Chronic enteric virus infection in two T-cell immunodeficient children. J Med Virol. 1988 Apr;24(4):435–444. doi: 10.1002/jmv.1890240410. [DOI] [PubMed] [Google Scholar]

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