Skip to main content
NCBI home page
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1995 Feb;114(1):123–132. doi: 10.1017/s0950268800051979

Molecular typing of Acinetobacter baumannii-Acinetobacter calcoaceticus complex isolates from endemic and epidemic nosocomial infections.

P Ratto 1, D O Sordelli 1, E Abeleira 1, M Torrero 1, M Catalano 1
PMCID: PMC2271339  PMID: 7867730

Abstract

Ribotype, biotype and resistance phenotype were used to characterize 37 Acinetobacter baumannii-A. calcoaceticus complex isolates responsible for nosocomial infections in Buenos Aires. Nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. By ribotyping isolates were classified into five different clones of A. baumannii biotype 2, 3 of A. baumannii biotype 9, and 3 of Acinetobacter genospecies 13. Combination of the three epidemiological markers permitted categorization of 18 outbreak isolates into four probable strains: 2 A. baumannii biotype 2, named type I, and II, and 2 A. baumannii biotype 9. Type I (15 isolates) was the most prevalent strain at one hospital and was responsible for the outbreak. In conclusion, combined analysis of biotypes, resistance phenotypes, and ribotypes was an accurate approach for epidemiologic investigation of A. baumannii. Furthermore, ribotyping discriminated Acinetobacter genospecies 13 isolates which were phenotypically difficult to type.

Full text

PDF
123

Images in this article

127Fig. 1
on p.127
128Fig. 2
on p.128

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews H. J. Acinetobacter bacteriocin typing. J Hosp Infect. 1986 Mar;7(2):169–175. doi: 10.1016/0195-6701(86)90060-5. [DOI] [PubMed] [Google Scholar]
  2. Bouvet P. J., Grimont P. A. Identification and biotyping of clinical isolates of Acinetobacter. Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):569–578. doi: 10.1016/0769-2609(87)90042-1. [DOI] [PubMed] [Google Scholar]
  3. Bouvet P. J., Jeanjean S. Delineation of new proteolytic genomic species in the genus Acinetobacter. Res Microbiol. 1989 May-Jun;140(4-5):291–299. doi: 10.1016/0923-2508(89)90021-1. [DOI] [PubMed] [Google Scholar]
  4. Bouvet P. J., Jeanjean S., Vieu J. F., Dijkshoorn L. Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains. J Clin Microbiol. 1990 Feb;28(2):170–176. doi: 10.1128/jcm.28.2.170-176.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crombach W. H., Dijkshoorn L., van Noort-Klaassen M., Niessen J., van Knippenberg-Gordebeke G. Control of an epidemic spread of a multi-resistant strain of Acinetobacter calcoaceticus in a hospital. Intensive Care Med. 1989;15(3):166–170. doi: 10.1007/BF01058568. [DOI] [PubMed] [Google Scholar]
  6. Dijkshoorn L., Aucken H. M., Gerner-Smidt P., Kaufmann M. E., Ursing J., Pitt T. L. Correlation of typing methods for Acinetobacter isolates from hospital outbreaks. J Clin Microbiol. 1993 Mar;31(3):702–705. doi: 10.1128/jcm.31.3.702-705.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Emori T. G., Gaynes R. P. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev. 1993 Oct;6(4):428–442. doi: 10.1128/cmr.6.4.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerner-Smidt P. Ribotyping of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J Clin Microbiol. 1992 Oct;30(10):2680–2685. doi: 10.1128/jcm.30.10.2680-2685.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gerner-Smidt P., Tjernberg I., Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol. 1991 Feb;29(2):277–282. doi: 10.1128/jcm.29.2.277-282.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gouby A., Carles-Nurit M. J., Bouziges N., Bourg G., Mesnard R., Bouvet P. J. Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii. J Clin Microbiol. 1992 Jun;30(6):1588–1591. doi: 10.1128/jcm.30.6.1588-1591.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gruner E., Kropec A., Huebner J., Altwegg M., Daschner F. Ribotyping of Pseudomonas aeruginosa strains isolated from surgical intensive care patients. J Infect Dis. 1993 May;167(5):1216–1220. doi: 10.1093/infdis/167.5.1216. [DOI] [PubMed] [Google Scholar]
  12. Joly-Guillou M. L., Bergogne-Berezin E., Vieu J. F. A study of the relationships between antibiotic resistance phenotypes, phage-typing and biotyping of 117 clinical isolates of Acinetobacter spp. J Hosp Infect. 1990 Jul;16(1):49–58. doi: 10.1016/0195-6701(90)90048-s. [DOI] [PubMed] [Google Scholar]
  13. Seifert H., Schulze A., Baginski R., Pulverer G. Comparison of four different methods for epidemiologic typing of Acinetobacter baumannii. J Clin Microbiol. 1994 Jul;32(7):1816–1819. doi: 10.1128/jcm.32.7.1816-1819.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Seifert H., Schulze A., Baginski R., Pulverer G. Plasmid DNA fingerprinting of Acinetobacter species other than Acinetobacter baumannii. J Clin Microbiol. 1994 Jan;32(1):82–86. doi: 10.1128/jcm.32.1.82-86.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tenover F. C., Arbeit R., Archer G., Biddle J., Byrne S., Goering R., Hancock G., Hébert G. A., Hill B., Hollis R. Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol. 1994 Feb;32(2):407–415. doi: 10.1128/jcm.32.2.407-415.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Traub W. H. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross-infection. J Clin Microbiol. 1989 Dec;27(12):2713–2716. doi: 10.1128/jcm.27.12.2713-2716.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vila J., Almela M., Jimenez de Anta M. T. Laboratory investigation of hospital outbreak caused by two different multiresistant Acinetobacter calcoaceticus subsp. anitratus strains. J Clin Microbiol. 1989 May;27(5):1086–1089. doi: 10.1128/jcm.27.5.1086-1089.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES