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. 1995 Oct;115(2):269–277. doi: 10.1017/s0950268800058398

PCR-based characterization of Yersinia enterocolitica: comparison with biotyping and serotyping.

P T Odinot 1, J F Meis 1, P J Van den Hurk 1, J A Hoogkamp-Korstanje 1, W J Melchers 1
PMCID: PMC2271416  PMID: 7589266

Abstract

PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discriminatory power. In comparisons with serotyping and biotyping, RAPD-fingerprinting was the most discriminatory technique and may therefore be a valuable epidemiological tool for the study of Y. enterocolitica infections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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