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. 1996 Jun;116(3):373–379. doi: 10.1017/s0950268800052705

An abattoir-based study of the prevalence of subclinical Johne's disease in adult cattle in south west England.

B Cetinkaya 1, K Egan 1, D A Harbour 1, K L Morgan 1
PMCID: PMC2271427  PMID: 8666083

Abstract

The prevalance of subclinical Johne's disease was estimated in adult cattle slaughtered at three major abattoirs in south west England. A polymerase chain reaction (PCR) based on IS900 was used to detect Mycobacterium paratuberculosis in intestinal lymph nodes of 1553 cattle. Culture was also carried out on all PCR positive and inconclusive samples. The prevalence of subclinical disease in adult cattle was 3.5% (95% confidence intervals (CI) 2.6-4.7) by PCR and 2.6% (CI 1.8-3.6) by culture. The proportion of the disease in each month ranged from 1.6% (CI 0.2-5.5) in April to 4.6% (CI 2.8-6.9) in November, but the difference was not significant (P > 0.05). The proportion of PCR positive lymph nodes in each abattoir ranged from 2.8% (CI 1.6-4.6) to 4.9% (CI 2.9-7.6), this difference was not significant either (P > 0.05). The prevalence in young cattle was 2.0% (CI 0.6-4.5). The difference between age groups was not statistically significant (P > 0.05).

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Selected References

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  1. Benedictus G., Dijkhuizen A. A., Stelwagen J. Economic losses due to paratuberculosis in dairy cattle. Vet Rec. 1987 Aug 15;121(7):142–146. doi: 10.1136/vr.121.7.142. [DOI] [PubMed] [Google Scholar]
  2. Buergelt C. D., Duncan J. R. Age and milk production data of cattle culled from a dairy herd with paratuberculosis. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 1):478–480. [PubMed] [Google Scholar]
  3. Cetinkaya B., Egan K., Morgan K. L. A practice-based survey of the frequency of Johne's disease in south west England. Vet Rec. 1994 May 7;134(19):494–497. doi: 10.1136/vr.134.19.494. [DOI] [PubMed] [Google Scholar]
  4. Challans J. A., Stevenson K., Reid H. W., Sharp J. M. A rapid method for the extraction and detection of Mycobacterium avium subspecies paratuberculosis from clinical specimens. Vet Rec. 1994 Jan 22;134(4):95–96. doi: 10.1136/vr.134.4.95. [DOI] [PubMed] [Google Scholar]
  5. Chiodini R. J., Van Kruiningen H. J., Merkal R. S. Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet. 1984 Jul;74(3):218–262. [PubMed] [Google Scholar]
  6. Chiodini R. J., Van Kruiningen H. J., Merkal R. S., Thayer W. R., Jr, Coutu J. A. Characteristics of an unclassified Mycobacterium species isolated from patients with Crohn's disease. J Clin Microbiol. 1984 Nov;20(5):966–971. doi: 10.1128/jcm.20.5.966-971.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chiodini R. J., van Kruiningen H. J. The prevalence of paratuberculosis in culled New England cattle. Cornell Vet. 1986 Jan;76(1):91–104. [PubMed] [Google Scholar]
  8. Green E. P., Tizard M. L., Moss M. T., Thompson J., Winterbourne D. J., McFadden J. J., Hermon-Taylor J. Sequence and characteristics of IS900, an insertion element identified in a human Crohn's disease isolate of Mycobacterium paratuberculosis. Nucleic Acids Res. 1989 Nov 25;17(22):9063–9073. doi: 10.1093/nar/17.22.9063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Merkal R. S., Larsen A. B., Booth G. D. Analysis of the effect of inapparent bovine paratuberculosis. Am J Vet Res. 1975 Jun;36(6):837–838. [PubMed] [Google Scholar]
  11. Merkal R. S., Whipple D. L., Sacks J. M., Snyder G. R. Prevalence of Mycobacterium paratuberculosis in ileocecal lymph nodes of cattle culled in the United States. J Am Vet Med Assoc. 1987 Mar 15;190(6):676–680. [PubMed] [Google Scholar]
  12. Morgan K. L. Johne's and Crohn's. Chronic inflammatory bowel diseases of infectious aetiology? Lancet. 1987 May 2;1(8540):1017–1019. doi: 10.1016/s0140-6736(87)92280-x. [DOI] [PubMed] [Google Scholar]
  13. Moss M. T., Sanderson J. D., Tizard M. L., Hermon-Taylor J., el-Zaatari F. A., Markesich D. C., Graham D. Y. Polymerase chain reaction detection of Mycobacterium paratuberculosis and Mycobacterium avium subsp silvaticum in long term cultures from Crohn's disease and control tissues. Gut. 1992 Sep;33(9):1209–1213. doi: 10.1136/gut.33.9.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Riemann H. P., Abbas B. Diagnosis and control of bovine paratuberculosis (Johne's disease). Adv Vet Sci Comp Med. 1983;27:481–506. [PubMed] [Google Scholar]
  15. SMITH H. W. The isolation of Mycobacteria from the mesenteric lymph-nodes of domestic animals. J Pathol Bacteriol. 1954 Oct;68(2):367–372. doi: 10.1002/path.1700680208. [DOI] [PubMed] [Google Scholar]
  16. Sanderson J. D., Moss M. T., Tizard M. L., Hermon-Taylor J. Mycobacterium paratuberculosis DNA in Crohn's disease tissue. Gut. 1992 Jul;33(7):890–896. doi: 10.1136/gut.33.7.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thoen C. O., Baum K. H. Current knowledge on paratuberculosis. J Am Vet Med Assoc. 1988 Jun 1;192(11):1609–1611. [PubMed] [Google Scholar]
  18. Vary P. H., Andersen P. R., Green E., Hermon-Taylor J., McFadden J. J. Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol. 1990 May;28(5):933–937. doi: 10.1128/jcm.28.5.933-937.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Whipple D. L., Kapke P. A., Andersen P. R. Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces. J Vet Diagn Invest. 1992 Jan;4(1):23–27. doi: 10.1177/104063879200400106. [DOI] [PubMed] [Google Scholar]
  20. van der Giessen J. W., Haring R. M., Vauclare E., Eger A., Haagsma J., van der Zeijst B. A. Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle: application in a control program. J Clin Microbiol. 1992 May;30(5):1216–1219. doi: 10.1128/jcm.30.5.1216-1219.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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