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. 1994 Apr;112(2):393–398. doi: 10.1017/s0950268800057800

A study of a hospital cluster of systemic candidosis using DNA typing methods.

F Romano 1, G Ribera 1, M Giuliano 1
PMCID: PMC2271449  PMID: 8150013

Abstract

A cluster of disseminated Candida albicans infections, which occurred at the Intensive Care Unit of the Department of Heart Surgery, was investigated. Ten patients became infected and seven died. A wide microbiological surveillance was carried out. A total of 14 isolates of Candida albicans, four environmental and ten human, were examined using the Restriction Endonuclease Analysis (REA) of DNA. The isolates were classified into five different main groups. Five of the clinical isolates had the predominant pattern Ab and two more clinical strains were very closely related. Two more isolates from the emergency kit desk and the hands of a nurse gave the same REA profile. Such a relationship proved the epidemic nature of the cluster, with most of the patients cross-infected, and strongly suggested transmission on the hands of the staff as a determinant of the epidemic. Thus, REA has the potential to address many important questions in the study of nosocomial epidemiology of Candida albicans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burnie J. P., Matthews R., Lee W., Philpott-Howard J., Brown R., Damani N., Breuer J., Honeywell K., Jordans Z. Four outbreaks of nosocomial systemic candidiasis. Epidemiol Infect. 1987 Aug;99(1):201–211. doi: 10.1017/s0950268800067030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burnie J. P., Odds F. C., Lee W., Webster C., Williams J. D. Outbreak of systemic Candida albicans in intensive care unit caused by cross infection. Br Med J (Clin Res Ed) 1985 Mar 9;290(6470):746–748. doi: 10.1136/bmj.290.6470.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carruba G., Pontieri E., De Bernardis F., Martino P., Cassone A. DNA fingerprinting and electrophoretic karyotype of environmental and clinical isolates of Candida parapsilosis. J Clin Microbiol. 1991 May;29(5):916–922. doi: 10.1128/jcm.29.5.916-922.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fox B. C., Mobley H. L., Wade J. C. The use of a DNA probe for epidemiological studies of candidiasis in immunocompromised hosts. J Infect Dis. 1989 Mar;159(3):488–494. doi: 10.1093/infdis/159.3.488. [DOI] [PubMed] [Google Scholar]
  5. Jordens J. Z. Restriction enzyme analysis of chromosomal DNA and its application in epidemiological studies. J Hosp Infect. 1991 Jun;18 (Suppl A):432–437. doi: 10.1016/0195-6701(91)90053-b. [DOI] [PubMed] [Google Scholar]
  6. Lee W., Burnie J. P., Matthews R. C., Oppenheim B. O., Damani N. N. Hospital outbreaks with yeasts. J Hosp Infect. 1991 Jun;18 (Suppl A):237–249. doi: 10.1016/0195-6701(91)90029-8. [DOI] [PubMed] [Google Scholar]
  7. Magee B. B., D'Souza T. M., Magee P. T. Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species. J Bacteriol. 1987 Apr;169(4):1639–1643. doi: 10.1128/jb.169.4.1639-1643.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Matthews R., Burnie J. Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis. BMJ. 1989 Feb 11;298(6670):354–357. doi: 10.1136/bmj.298.6670.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Merz W. G. Candida albicans strain delineation. Clin Microbiol Rev. 1990 Oct;3(4):321–334. doi: 10.1128/cmr.3.4.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oren I., Manavathu E. K., Lerner S. A. Isolation and characterization of a species-specific DNA probe for Candida albicans. Nucleic Acids Res. 1991 Dec;19(25):7113–7116. doi: 10.1093/nar/19.25.7113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pfaller M. A. The use of molecular techniques for epidemiologic typing of Candida species. Curr Top Med Mycol. 1992;4:43–63. doi: 10.1007/978-1-4612-2762-5_2. [DOI] [PubMed] [Google Scholar]
  12. Scherer S., Magee P. T. Genetics of Candida albicans. Microbiol Rev. 1990 Sep;54(3):226–241. doi: 10.1128/mr.54.3.226-241.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scherer S., Stevens D. A. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987 Apr;25(4):675–679. doi: 10.1128/jcm.25.4.675-679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stevens D. A., Odds F. C., Scherer S. Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype. Rev Infect Dis. 1990 Mar-Apr;12(2):258–266. doi: 10.1093/clinids/12.2.258. [DOI] [PubMed] [Google Scholar]
  15. Warnock D. W. Typing of Candida albicans. J Hosp Infect. 1984 Sep;5(3):244–252. doi: 10.1016/0195-6701(84)90073-2. [DOI] [PubMed] [Google Scholar]
  16. Wilkinson B. M., Morris L., Adams D. J., Evans E. G., Lacey C. J., Walmsley R. M. A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system. J Med Vet Mycol. 1992;30(2):123–131. [PubMed] [Google Scholar]

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