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. 1994 Jun;112(3):473–480. doi: 10.1017/s0950268800051177

Apramycin-resistant Escherichia coli isolated from pigs and a stockman.

J E Hunter 1, M Bennett 1, C A Hart 1, J C Shelley 1, J R Walton 1
PMCID: PMC2271502  PMID: 8005213

Abstract

Escherichia coli serotype O147:K89:K88a,c was found to be associated with outbreaks of diarrhoea in preweaner pigs of up to 4 weeks of age on a pig unit. Resistance to apramycin, gentamicin, netilmicin, tobramycin and other antibiotics was associated with conjugative plasmids of approximately 62 kb. The presence of a gene which encoded for the aminoglycoside acetyltransferase enzyme AAC(3)IV was confirmed by DNA hybridization. Samples collected during the following 12 months revealed widespread dissemination of these resistance plasmids in non-serotypable, non-haemolytic E. coli throughout the farm. Apramycin-resistant E. coli were also isolated from a stockman and it appeared from plasmid profile analysis and antibiotic sensitivity testing that the human isolates carried the same plasmid as that carried by the porcine E. coli. Klebsiella pneumoniae, with a slightly smaller conjugative plasmid and similar resistance pattern, was isolated from the stockman's wife.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bräu B., Pilz U., Piepersberg W. Genes for gentamicin-(3)-N-acetyltransferases III and IV: I. Nucleotide sequence of the AAC(3)-IV gene and possible involvement of an IS140 element in its expression. Mol Gen Genet. 1984;193(1):179–187. doi: 10.1007/BF00327434. [DOI] [PubMed] [Google Scholar]
  2. Chaslus-Dancla E., Glupcznski Y., Gerbaud G., Lagorce M., Lafont J. P., Courvalin P. Detection of apramycin resistant Enterobacteriaceae in hospital isolates. FEMS Microbiol Lett. 1989 Oct 15;52(3):261–265. doi: 10.1016/0378-1097(89)90208-5. [DOI] [PubMed] [Google Scholar]
  3. Chaslus-Dancla E., Martel J. L., Carlier C., Lafont J. P., Courvalin P. Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France. Antimicrob Agents Chemother. 1986 Feb;29(2):239–243. doi: 10.1128/aac.29.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duff J. P., Hunt B. W. Lambs die from porcine E coli. Vet Rec. 1989 Oct 7;125(15):404–404. doi: 10.1136/vr.125.15.404-a. [DOI] [PubMed] [Google Scholar]
  5. Gellin G., Langlois B. E., Dawson K. A., Aaron D. K. Antibiotic resistance of gram-negative enteric bacteria from pigs in three herds with different histories of antibiotic exposure. Appl Environ Microbiol. 1989 Sep;55(9):2287–2292. doi: 10.1128/aem.55.9.2287-2292.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hunter J. E., Hart C. A., Shelley J. C., Walton J. R., Bennett M. Human isolates of apramycin-resistant Escherichia coli which contain the genes for the AAC(3)IV enzyme. Epidemiol Infect. 1993 Apr;110(2):253–259. doi: 10.1017/s0950268800068175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hunter J. E., Shelley J. C., Walton J. R., Hart C. A., Bennett M. Apramycin resistance plasmids in Escherichia coli: possible transfer to Salmonella typhimurium in calves. Epidemiol Infect. 1992 Apr;108(2):271–278. doi: 10.1017/s0950268800049748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jørgensen S. T., Sørensen V. W. Spread of an R plasmid among antigen types of Escherichia coli pathogenic for piglets. Plasmid. 1979 Apr;2(2):290–292. doi: 10.1016/0147-619x(79)90047-7. [DOI] [PubMed] [Google Scholar]
  9. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Langlois B. E., Cromwell G. L., Stahly T. S., Dawson K. A., Hays V. W. Antibiotic resistance of fecal coliforms after long-term withdrawal of therapeutic and subtherapeutic antibiotic use in a swine herd. Appl Environ Microbiol. 1983 Dec;46(6):1433–1434. doi: 10.1128/aem.46.6.1433-1434.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mee B. J., Nikoletti S. M. Plasmids encoding trimethoprim resistance in bacterial isolates from man and pigs. J Appl Bacteriol. 1983 Apr;54(2):225–235. doi: 10.1111/j.1365-2672.1983.tb02611.x. [DOI] [PubMed] [Google Scholar]
  12. Threlfall E. J., Rowe B., Ferguson J. L., Ward L. R. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg (Lond) 1986 Dec;97(3):419–426. doi: 10.1017/s0022172400063609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wray C., Hedges R. W., Shannon K. P., Bradley D. E. Apramycin and gentamicin resistance in Escherichia coli and salmonellas isolated from farm animals. J Hyg (Lond) 1986 Dec;97(3):445–456. doi: 10.1017/s0022172400063622. [DOI] [PMC free article] [PubMed] [Google Scholar]

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