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. 1994 Oct;113(2):377–385. doi: 10.1017/s0950268800051803

Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep.

P Chand 1, R P Kitching 1, D N Black 1
PMCID: PMC2271533  PMID: 7925674

Abstract

This paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturally-acquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 26 kDa proteins of capripoxvirus provided a firm basis for differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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