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. 1996 Aug;117(1):59–67. doi: 10.1017/s0950268800001138

Detection of pathogenic Yersinia enterocolitica using the multiplex polymerase chain reaction.

N Harnett 1, Y P Lin 1, C Krishnan 1
PMCID: PMC2271660  PMID: 8760951

Abstract

A multiplex polymerase chain reaction (PCR) was developed to detect the presence of the ail, yst, and virF genes of Yersinia enterocolitica simultaneously, quickly and accurately. The amplified fragment sizes were 356 base-pairs (bp) for the ail gene, 134 bp for the yst gene, and 231 bp for the virF gene. The specificity of the amplified products was confirmed by hybridization with digoxigenin-labelled oligonucleotide probes. Amplification was successful whether the template was derived from a single colony of bacteria, aliquots of boiled bacterial suspensions, from DNA extracted from pure or mixed cultures or from stool specimens. Amplification of the virF gene was also achieved from strains of Y. pseudotuberculosis carrying the 70 kb plasmid but not with preparations from other related Yersinia species or from other members of the family Enterobacteriaceae. The detection limit we established was 5-10 colony forming units per millilitre (cfu/ml) and 1.0 pg of DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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