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. 1992 Oct;109(2):227–239. doi: 10.1017/s0950268800050184

Phylogenetic and epidemiological analysis of Neisseria meningitidis using DNA probes.

H Ni 1, A I Knight 1, K A Cartwright 1, J J McFadden 1
PMCID: PMC2271919  PMID: 1356820

Abstract

The genetic relationships between various serotypes and serogroups of meningococcal strains were investigated by restriction fragment-length polymorphism (RFLP) analysis using a number of random DNA probes and a probe containing a truncated copy of the meningococcal insertion sequence IS1106. The data were used to estimate genetic distance between all pairs of strains and to construct phylogenetic trees for meningococcal strains. B15:P1.16R strains isolated from cases of systemic meningococcal disease in two health districts with a high incidence of disease were clonal in contrast to similar strains from cases occurring in other parts of the UK. Strains from these areas, which contain a similar genomic deletion, were found to be derived from two distinct lineages within the B15:P1.16R phylogenetic group. RFLP data demonstrated that present serological typing systems for the meningococcus do not necessarily reflect true genetic relationships.

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Selected References

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