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. 1992 Dec;109(3):423–432. doi: 10.1017/s095026880005041x

A study of phenotypic variation of Staphylococcus epidermidis using Congo red agar.

M A Deighton 1, J Capstick 1, R Borland 1
PMCID: PMC2271935  PMID: 1468527

Abstract

This study examines a series of phenotypic variants of Staphylococcus epidermidis that were generated from a pair of parent variants, isolated from valvular tissue of a patient with prosthetic valve endocarditis. The variants were initially classified by examining their colonial morphology on Congo red agar. In addition to differences in Congo red binding and colonial morphology, they differed in the expression of several surface components and enzymes. Despite these phenotypic differences, all variants had the same restriction endonuclease profile of plasmid DNA. Examination of a collection of clinical isolates demonstrated that phenotypic variation is a common property of S. epidermidis. The ability to express different combinations of surface components and enzymes could contribute to the virulence of S. epidermidis strains by enabling these organisms to colonize a range of diverse environments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baddour L. M., Barker L. P., Christensen G. D., Parisi J. T., Simpson W. A. Phenotypic variation of Staphylococcus epidermidis in infection of transvenous endocardial pacemaker electrodes. J Clin Microbiol. 1990 Apr;28(4):676–679. doi: 10.1128/jcm.28.4.676-679.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baddour L. M., Christensen G. D., Lowrance J. H., Simpson W. A. Pathogenesis of experimental endocarditis. Rev Infect Dis. 1989 May-Jun;11(3):452–463. doi: 10.1093/clinids/11.3.452. [DOI] [PubMed] [Google Scholar]
  3. Baddour L. M., Christensen G. D. Prosthetic valve endocarditis due to small-colony staphylococcal variants. Rev Infect Dis. 1987 Nov-Dec;9(6):1168–1174. doi: 10.1093/clinids/9.6.1168. [DOI] [PubMed] [Google Scholar]
  4. Baddour L. M., Phillips T. N., Bisno A. L. Coagulase-negative staphylococcal endocarditis. Occurrence in patients with mitral valve prolapse. Arch Intern Med. 1986 Jan;146(1):119–121. doi: 10.1001/archinte.146.1.119. [DOI] [PubMed] [Google Scholar]
  5. Baddour L. M., Simpson W. A., Weems J. J., Jr, Hill M. M., Christensen G. D. Phenotypic selection of small-colony variant forms of Staphylococcus epidermidis in the rat model of endocarditis. J Infect Dis. 1988 Apr;157(4):757–763. doi: 10.1093/infdis/157.4.757. [DOI] [PubMed] [Google Scholar]
  6. Borst P., Greaves D. R. Programmed gene rearrangements altering gene expression. Science. 1987 Feb 6;235(4789):658–667. doi: 10.1126/science.3544215. [DOI] [PubMed] [Google Scholar]
  7. Christensen G. D., Baddour L. M., Madison B. M., Parisi J. T., Abraham S. N., Hasty D. L., Lowrance J. H., Josephs J. A., Simpson W. A. Colonial morphology of staphylococci on Memphis agar: phase variation of slime production, resistance to beta-lactam antibiotics, and virulence. J Infect Dis. 1990 Jun;161(6):1153–1169. doi: 10.1093/infdis/161.6.1153. [DOI] [PubMed] [Google Scholar]
  8. Christensen G. D., Baddour L. M., Simpson W. A. Phenotypic variation of Staphylococcus epidermidis slime production in vitro and in vivo. Infect Immun. 1987 Dec;55(12):2870–2877. doi: 10.1128/iai.55.12.2870-2877.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Christensen G. D., Simpson W. A., Bisno A. L., Beachey E. H. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun. 1982 Jul;37(1):318–326. doi: 10.1128/iai.37.1.318-326.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Christensen G. D., Simpson W. A., Younger J. J., Baddour L. M., Barrett F. F., Melton D. M., Beachey E. H. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 1985 Dec;22(6):996–1006. doi: 10.1128/jcm.22.6.996-1006.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Deighton M. A., Balkau B. Adherence measured by microtiter assay as a virulence marker for Staphylococcus epidermidis infections. J Clin Microbiol. 1990 Nov;28(11):2442–2447. doi: 10.1128/jcm.28.11.2442-2447.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Deighton M., Pearson S., Capstick J., Spelman D., Borland R. Phenotypic variation of Staphylococcus epidermidis isolated from a patient with native valve endocarditis. J Clin Microbiol. 1992 Sep;30(9):2385–2390. doi: 10.1128/jcm.30.9.2385-2390.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Finlay B. B., Falkow S. Common themes in microbial pathogenicity. Microbiol Rev. 1989 Jun;53(2):210–230. doi: 10.1128/mr.53.2.210-230.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Franson T. R., Sheth N. K., Menon L., Sohnle P. G. Persistent in vitro survival of coagulase-negative staphylococci adherent to intravascular catheters in the absence of conventional nutrients. J Clin Microbiol. 1986 Oct;24(4):559–561. doi: 10.1128/jcm.24.4.559-561.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Freeman D. J., Falkiner F. R., Keane C. T. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol. 1989 Aug;42(8):872–874. doi: 10.1136/jcp.42.8.872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gemmell C. G., Roberts E. Toxins and enzymes of coagulase negative staphylococci isolated from human infections. J Hyg Epidemiol Microbiol Immunol. 1974;18(3):276–280. [PubMed] [Google Scholar]
  17. Gristina A. G. Biomaterial-centered infection: microbial adhesion versus tissue integration. Science. 1987 Sep 25;237(4822):1588–1595. doi: 10.1126/science.3629258. [DOI] [PubMed] [Google Scholar]
  18. Lyon B. R., May J. W., Skurray R. A. Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1983 Jun;23(6):817–826. doi: 10.1128/aac.23.6.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schwalbe R. S., Stapleton J. T., Gilligan P. H. Emergence of vancomycin resistance in coagulase-negative staphylococci. N Engl J Med. 1987 Apr 9;316(15):927–931. doi: 10.1056/NEJM198704093161507. [DOI] [PubMed] [Google Scholar]
  20. Travis L. B., MacLowry J. D. Clinically significant differences in antibiograms of morphologic variants of blood culture isolates. Diagn Microbiol Infect Dis. 1989 Mar-Apr;12(2):177–179. doi: 10.1016/0732-8893(89)90010-2. [DOI] [PubMed] [Google Scholar]
  21. Woods D. E., Sokol P. A., Bryan L. E., Storey D. G., Mattingly S. J., Vogel H. J., Ceri H. In vivo regulation of virulence in Pseudomonas aeruginosa associated with genetic rearrangement. J Infect Dis. 1991 Jan;163(1):143–149. doi: 10.1093/infdis/163.1.143. [DOI] [PubMed] [Google Scholar]

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