Abstract
A new technique to study the prevalence of circulating antibodies directed against different morphological groups ('morphotypes') of bacteria in fresh faeces is presented. The technique combines quantitative indirect immunofluorescence with digital image analysis. Plasma antibody titres and patterns of IgA, IgG and IgM isotype against morphotypes of faecal bacteria were determined in ten healthy individuals.
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Selected References
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- Apperloo-Renkema H. Z., Wilkinson M. H., Oenema D. G., van der Waaij D. Objective quantitation of serum antibody titres against Enterobacteriaceae using indirect immunofluorescence, read by videocamera and image processing system. Med Microbiol Immunol. 1991;180(2):93–100. doi: 10.1007/BF00193850. [DOI] [PubMed] [Google Scholar]
- Evans M. E., Pollack M., Hardegen N. J., Koles N. L., Guelde G., Chia J. K. Fluorescence-activated cell sorter analysis of binding by lipopolysaccharide-specific monoclonal antibodies to gram-negative bacteria. J Infect Dis. 1990 Jul;162(1):148–155. doi: 10.1093/infdis/162.1.148. [DOI] [PubMed] [Google Scholar]
- Lieberum B., Hartmann K. U. Successive changes of the cellular composition in lymphoid organs of MRL-Mp/lpr-lpr mice during the development of lymphoproliferative disease as investigated in cryosections. Clin Immunol Immunopathol. 1988 Mar;46(3):421–431. doi: 10.1016/0090-1229(88)90061-x. [DOI] [PubMed] [Google Scholar]
- Meijer B. C., Kootstra G. J., Wilkinson M. H. Morphometrical parameters of gut microflora in human volunteers. Epidemiol Infect. 1991 Oct;107(2):383–391. doi: 10.1017/s0950268800049025. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Savage D. C. Microbial ecology of the gastrointestinal tract. Annu Rev Microbiol. 1977;31:107–133. doi: 10.1146/annurev.mi.31.100177.000543. [DOI] [PubMed] [Google Scholar]
- Shapiro P. E., McAllister G., Lerner E. A., Lerner M. R. Healthy individuals and patients with systemic lupus erythematosus have unique, person-specific spectra of antibodies detectable on immunoblots. Clin Immunol Immunopathol. 1991 Apr;59(1):129–138. doi: 10.1016/0090-1229(91)90087-q. [DOI] [PubMed] [Google Scholar]
- Van de Merwe J. P., Stegeman J. H., Hazenberg M. P. The resident faecal flora is determined by genetic characteristics of the host. Implications for Crohn's disease? Antonie Van Leeuwenhoek. 1983 Jun;49(2):119–124. doi: 10.1007/BF00393669. [DOI] [PubMed] [Google Scholar]
- Veenendaal D., de Boer F., Van der Waaij D. Effect of selective decontamination of the digestive tract of donor and recipient on the occurrence of murine delayed-type graft-versus-host disease. Med Microbiol Immunol. 1988;177(3):133–144. doi: 10.1007/BF00232893. [DOI] [PubMed] [Google Scholar]