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. 1992 Apr;108(2):213–220. doi: 10.1017/s0950268800049694

Genetic relationships among strains of Salmonella enteritidis in a national epidemic in Switzerland.

J Stanley 1, A P Burnens 1, E J Threlfall 1, N Chowdry 1, M Goldsworthy 1
PMCID: PMC2271979  PMID: 1316285

Abstract

A collection of Salmonella enteritidis strains isolated in Switzerland (1965-90) was characterized. The phage type and plasmid profile of isolates were compared with the copy number and insertion loci of the DNA insertion element IS200. Three clonal lines of S. enteritidis were identified by IS200 profile; the various phage types were subtypes reproducibly associated with one of these lines. All human and poultry isolates contained a 38 Mda plasmid which hybridized with a mouse virulence-associated gene probe. In S. enteritidis, the IS200 profile is a race-specific molecular marker of the chromosome, and may be particularly applicable for studying the epidemiology of less common serovars.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chart H., Rowe B. Antibodies to lipopolysaccharide and outer membrane proteins of Salmonella enteritidis PT4 are not involved in protection from experimental infection. FEMS Microbiol Lett. 1991 Dec 1;68(3):345–350. doi: 10.1016/0378-1097(91)90380-s. [DOI] [PubMed] [Google Scholar]
  2. Grannum P. A., Ghidini A., Scioscia A., Copel J. A., Romero R., Hobbins J. C. Assessment of fetal renal reserve in low level obstructive uropathy. Lancet. 1989 Feb 4;1(8632):281–282. doi: 10.1016/s0140-6736(89)91296-8. [DOI] [PubMed] [Google Scholar]
  3. Humphrey T. J., Mead G. C., Rowe B. Poultry meat as a source of human salmonellosis in England and Wales. Epidemiological overview. Epidemiol Infect. 1988 Apr;100(2):175–184. doi: 10.1017/s0950268800067303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lam S., Roth J. R. IS200: a Salmonella-specific insertion sequence. Cell. 1983 Oct;34(3):951–960. doi: 10.1016/0092-8674(83)90552-4. [DOI] [PubMed] [Google Scholar]
  6. Martinetti G., Altwegg M. rRNA gene restriction patterns and plasmid analysis as a tool for typing Salmonella enteritidis. Res Microbiol. 1990 Nov-Dec;141(9):1151–1162. doi: 10.1016/0923-2508(90)90088-8. [DOI] [PubMed] [Google Scholar]
  7. Platt D. J., Taggart J., Heraghty K. A. Molecular divergence of the serotype-specific plasmid (pSLT) among strains of Salmonella typhimurium of human and veterinary origin and comparison of pSLT with the serotype specific plasmids of S. enteritidis and S. dublin. J Med Microbiol. 1988 Dec;27(4):277–284. doi: 10.1099/00222615-27-4-277. [DOI] [PubMed] [Google Scholar]
  8. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  9. Spies U., Müller H., Becht H. Nucleotide sequence of infectious bursal disease virus genome segment A delineates two major open reading frames. Nucleic Acids Res. 1989 Oct 11;17(19):7982–7982. doi: 10.1093/nar/17.19.7982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. St Louis M. E., Morse D. L., Potter M. E., DeMelfi T. M., Guzewich J. J., Tauxe R. V., Blake P. A. The emergence of grade A eggs as a major source of Salmonella enteritidis infections. New implications for the control of salmonellosis. JAMA. 1988 Apr 8;259(14):2103–2107. [PubMed] [Google Scholar]
  11. Ward L. R., de Sa J. D., Rowe B. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect. 1987 Oct;99(2):291–294. doi: 10.1017/s0950268800067765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Williamson C. M., Baird G. D., Manning E. J. A common virulence region on plasmids from eleven serotypes of Salmonella. J Gen Microbiol. 1988 Apr;134(4):975–982. doi: 10.1099/00221287-134-4-975. [DOI] [PubMed] [Google Scholar]
  13. Woodward M. J., McLaren I., Wray C. Distribution of virulence plasmids within Salmonellae. J Gen Microbiol. 1989 Mar;135(3):503–511. doi: 10.1099/00221287-135-3-503. [DOI] [PubMed] [Google Scholar]

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