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. 1991 Apr;106(2):289–295. doi: 10.1017/s0950268800048433

Endotoxin liberation by strains of N. meningitidis isolated from patients and healthy carriers.

M C Mellado 1, R Rodríguez-Contreras 1, M Fernandez-Crehuet 1, R Lopez-Gigosos 1, M Delgado Rodriguez 1, R Galvez-Vargas 1
PMCID: PMC2272015  PMID: 1902183

Abstract

The main objective of this study was to assess whether the capacity of Neisseria meningitidis to release endotoxin depends upon the type of strain or upon bacterial mass. Endotoxin release was studied in 32 strains isolated from patients with meningococcal infections and in 49 from asymptomatic carriers, using a quantitative test (limulus test with a chromogenic substrate). The results show that the strains from patients release significantly higher amounts of endotoxin than strains from carriers regardless of serogroup and isolation site. No correlation was found between stage of bacterial growth and the amount of endotoxin liberated. These findings suggest that endotoxin liberation is a characteristic of certain strains of N. meningitidis and is not determined simply by bacterial mass.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen B. M. Disease and mortality caused by Neisseria meningitidis. The role of endotoxin liberation as a virulence factor. J Oslo City Hosp. 1983 Apr-May;33(4-5):37–68. [PubMed] [Google Scholar]
  2. Andersen B. M., Solberg O. Endotoxin liberation and invasivity of Neisseria meningitidis. Scand J Infect Dis. 1984;16(3):247–254. doi: 10.3109/00365548409070397. [DOI] [PubMed] [Google Scholar]
  3. Andersen B. M., Solberg O. Endotoxin liberation associated with growth, encapsulation and virulence of Neisseria meningitidis. Scand J Infect Dis. 1988;20(1):21–31. doi: 10.3109/00365548809117213. [DOI] [PubMed] [Google Scholar]
  4. Andersen B. M., Solberg O. Liberation of endotoxin during growth of Neisseria meningitidis in a chemically-defined medium. Acta Pathol Microbiol Scand B. 1978 Oct;86B(5):275–281. doi: 10.1111/j.1699-0463.1978.tb00044.x. [DOI] [PubMed] [Google Scholar]
  5. Dwelle T. L., Dunkle L. M., Blair L. Correlation of cerebrospinal fluid endotoxinlike activity with clinical and laboratory variables in gram-negative bacterial meningitis in children. J Clin Microbiol. 1987 May;25(5):856–858. doi: 10.1128/jcm.25.5.856-858.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harthug S., Bjorvatn B., Osterud B. Quantitation of endotoxin in blood from patients with meningococcal disease using a limulus lysate test in combination with chromogenic substrate. Infection. 1983 Jul-Aug;11(4):192–195. doi: 10.1007/BF01641194. [DOI] [PubMed] [Google Scholar]
  7. Hawiger J., Hawiger A., Steckley S., Timmons S., Cheng C. Membrane changes in human platelets induced by lipopolysaccharide endotoxin. Br J Haematol. 1977 Feb;35(2):285–299. doi: 10.1111/j.1365-2141.1977.tb00585.x. [DOI] [PubMed] [Google Scholar]
  8. Holbein B. E. Enhancement of Neisseria meningitidis infection in mice by addition of iron bound to transferrin. Infect Immun. 1981 Oct;34(1):120–125. doi: 10.1128/iai.34.1.120-125.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tubbs H. R. Endotoxin in meningococcal infections. Arch Dis Child. 1980 Oct;55(10):808–810. doi: 10.1136/adc.55.10.808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Zollinger W. D., Kasper D. L., Veltri B. J., Artenstein M. S. Isolation and characterization of a native cell wall complex from Neisseria meningitidis. Infect Immun. 1972 Nov;6(5):835–851. doi: 10.1128/iai.6.5.835-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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