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. 1991 Oct;107(2):405–410. doi: 10.1017/s0950268800049049

A dot-blot hybridization procedure for the detection of astrovirus in stool samples.

M M Willcocks 1, M J Carter 1, J G Silcock 1, C R Madeley 1
PMCID: PMC2272075  PMID: 1718770

Abstract

We have developed a nucleic acid dot-blot hybridization test for the detection of astroviruses in stool samples. The test was not as sensitive as electron microscopy for the detection of low numbers of well preserved astrovirus particles, but was able to identify astroviruses in stools containing particles of indistinct morphology. In total, this procedure identified astroviruses in more samples than did electron microscopy, and the data indicate that the incidence of astroviruses may be substantially underestimated.

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Selected References

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  1. Barrett T., Wolstenholme A. J., Mahy B. W. Transcription and replication of influenza virus RNA. Virology. 1979 Oct 15;98(1):211–225. doi: 10.1016/0042-6822(79)90539-7. [DOI] [PubMed] [Google Scholar]
  2. Caul E. O., Appleton H. The electron microscopical and physical characteristics of small round human fecal viruses: an interim scheme for classification. J Med Virol. 1982;9(4):257–265. doi: 10.1002/jmv.1890090403. [DOI] [PubMed] [Google Scholar]
  3. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  4. Herrmann J. E., Hudson R. W., Perron-Henry D. M., Kurtz J. B., Blacklow N. R. Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. J Infect Dis. 1988 Jul;158(1):182–185. doi: 10.1093/infdis/158.1.182. [DOI] [PubMed] [Google Scholar]
  5. Herrmann J. E., Nowak N. A., Perron-Henry D. M., Hudson R. W., Cubitt W. D., Blacklow N. R. Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies. J Infect Dis. 1990 Feb;161(2):226–229. doi: 10.1093/infdis/161.2.226. [DOI] [PubMed] [Google Scholar]
  6. Kurtz J. B., Lee T. W. Human astrovirus serotypes. Lancet. 1984 Dec 15;2(8416):1405–1405. doi: 10.1016/s0140-6736(84)92101-9. [DOI] [PubMed] [Google Scholar]
  7. Lew J. F., Glass R. I., Petric M., Lebaron C. W., Hammond G. W., Miller S. E., Robinson C., Boutilier J., Riepenhoff-Talty M., Payne C. M. Six-year retrospective surveillance of gastroenteritis viruses identified at ten electron microscopy centers in the United States and Canada. Pediatr Infect Dis J. 1990 Oct;9(10):709–714. doi: 10.1097/00006454-199010000-00005. [DOI] [PubMed] [Google Scholar]
  8. Madeley C. R., Cosgrove B. P. Letter: 28 nm particles in faeces in infantile gastroenteritis. Lancet. 1975 Sep 6;2(7932):451–452. doi: 10.1016/s0140-6736(75)90858-2. [DOI] [PubMed] [Google Scholar]
  9. Oliver A. R., Phillips A. D. An electron microscopical investigation of faecal small round viruses. J Med Virol. 1988 Feb;24(2):211–218. doi: 10.1002/jmv.1890240211. [DOI] [PubMed] [Google Scholar]
  10. Oshiro L. S., Haley C. E., Roberto R. R., Riggs J. L., Croughan M., Greenberg H., Kapikian A. A 27-nm virus isolated during an outbreak of acute infectious nonbacterial gastroenteritis in a convalescent hospital: a possible new serotype. J Infect Dis. 1981 Jun;143(6):791–795. doi: 10.1093/infdis/143.6.791. [DOI] [PubMed] [Google Scholar]
  11. Willcocks M. M., Carter M. J., Laidler F. R., Madeley C. R. Growth and characterisation of human faecal astrovirus in a continuous cell line. Arch Virol. 1990;113(1-2):73–81. doi: 10.1007/BF01318354. [DOI] [PubMed] [Google Scholar]

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