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. 1992 Jun;108(3):555–557. doi: 10.1017/s0950268800050056

Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain.

C Herrero 1, C Pelaz 1, C Martín-Bourgon 1
PMCID: PMC2272212  PMID: 1601085

Abstract

Ticks recovered from dogs in rural villages around Madrid (Spain) were processed to isolate rickettsiae. One sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive to Rickettsia conorii, were decontaminated, homogenized and inoculated onto Vero cells. Two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. One sample (14H) was successfully subcultured and identified as a member of the spotted fever group rickettsia. Tick eggs provide suitable material for isolation of rickettsia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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