Skip to main content
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1992 Jun;108(3):463–467. doi: 10.1017/s0950268800049979

Analysis of some virulence factors of Vibrio vulnificus isolated from Rio de Janeiro, Brazil.

D P Rodrigues 1, R V Ribeiro 1, E Hofer 1
PMCID: PMC2272213  PMID: 1601080

Abstract

Twenty strains of V. vulnificus isolated from the environment were investigated for characteristics related to their infectivity such as colonial morphology, enzymatic activity and animal assays. The presence of DNase, chitinase, amylase, lecithinase and gelatinase was observed in 100% of the strains, haemolytic activity was absent, and variable results were obtained in elastase, collagenase and chondroitinase. In the animal assays, 70% of the strains were lethal to adult mice, while 45% caused fluid accumulation in suckling mice. Although all strains had opaque colonies, only 3 of the 20 had the three enzymes elastase, collagenase and gelatinase, and only one of these was virulent in animal assays.

Full text

PDF
463

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowdre J. H., Poole M. D., Oliver J. D. Edema and hemoconcentration in mice experimentally infected with Vibrio vulnificus. Infect Immun. 1981 Jun;32(3):1193–1199. doi: 10.1128/iai.32.3.1193-1199.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gander R. M., LaRocco M. T. Detection of piluslike structures on clinical and environmental isolates of Vibrio vulnificus. J Clin Microbiol. 1989 May;27(5):1015–1021. doi: 10.1128/jcm.27.5.1015-1021.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gray L. D., Kreger A. S. Purification and characterization of an extracellular cytolysin produced by Vibrio vulnificus. Infect Immun. 1985 Apr;48(1):62–72. doi: 10.1128/iai.48.1.62-72.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Janda J. M., Powers C., Bryant R. G., Abbott S. L. Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin Microbiol Rev. 1988 Jul;1(3):245–267. doi: 10.1128/cmr.1.3.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaysner C. A., Abeyta C., Jr, Wekell M. M., DePaola A., Jr, Stott R. F., Leitch J. M. Virulent strains of Vibrio vulnificus isolated from estuaries of the United States West Coast. Appl Environ Microbiol. 1987 Jun;53(6):1349–1351. doi: 10.1128/aem.53.6.1349-1351.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kelly M. T. Effect of temperature and salinity on Vibrio (Beneckea) vulnificus occurrence in a Gulf Coast environment. Appl Environ Microbiol. 1982 Oct;44(4):820–824. doi: 10.1128/aem.44.4.820-824.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klontz K. C., Lieb S., Schreiber M., Janowski H. T., Baldy L. M., Gunn R. A. Syndromes of Vibrio vulnificus infections. Clinical and epidemiologic features in Florida cases, 1981-1987. Ann Intern Med. 1988 Aug 15;109(4):318–323. doi: 10.7326/0003-4819-109-4-318. [DOI] [PubMed] [Google Scholar]
  8. Kothary M. H., Kreger A. S. Purification and characterization of an elastolytic protease of Vibrio vulnificus. J Gen Microbiol. 1987 Jul;133(7):1783–1791. doi: 10.1099/00221287-133-7-1783. [DOI] [PubMed] [Google Scholar]
  9. Kreger A., Lockwood D. Detection of extracellular toxin(s) produced by Vibrio vulnificus. Infect Immun. 1981 Aug;33(2):583–590. doi: 10.1128/iai.33.2.583-590.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morris J. G., Jr, Black R. E. Cholera and other vibrioses in the United States. N Engl J Med. 1985 Feb 7;312(6):343–350. doi: 10.1056/NEJM198502073120604. [DOI] [PubMed] [Google Scholar]
  11. Nishibuchi M., Seidler R. J. Medium-dependent production of extracellular enterotoxins by non-O-1 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvialis. Appl Environ Microbiol. 1983 Jan;45(1):228–231. doi: 10.1128/aem.45.1.228-231.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Simpson L. M., White V. K., Zane S. F., Oliver J. D. Correlation between virulence and colony morphology in Vibrio vulnificus. Infect Immun. 1987 Jan;55(1):269–272. doi: 10.1128/iai.55.1.269-272.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith G. C., Merkel J. R. Collagenolytic activity of Vibrio vulnificus: potential contribution to its invasiveness. Infect Immun. 1982 Mar;35(3):1155–1156. doi: 10.1128/iai.35.3.1155-1156.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thorpe T. C., Miller R. D. Extracellular enzymes of Legionella pneumophila. Infect Immun. 1981 Aug;33(2):632–635. doi: 10.1128/iai.33.2.632-635.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tison D. L., Kelly M. T. Virulence of Vibrio vulnificus strains from marine environments. Appl Environ Microbiol. 1986 May;51(5):1004–1006. doi: 10.1128/aem.51.5.1004-1006.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. West P. A. The human pathogenic vibrios--a public health update with environmental perspectives. Epidemiol Infect. 1989 Aug;103(1):1–34. doi: 10.1017/s0950268800030326. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES